Effects of Estrodiol and Progestrone on Epilepsy in Chemical Kindling Model in Rats

 M.Khaksari, E. Rezvani, M.A. Sajjadi, F.Hasanshahie Department of physiology, Rafsanjan Medical Faculty, Rafsanjan, Iran Background: Since, 30-40 percent of epileptic patient don’t reponse to current therapy or be low responsive patient after long-term treatment, then finding of new drug paradigms is ncessarily. Recesnty, some studies indicated about sex steroids that modulate GABA activity, that is the aim of this study to determine the effect of estradiol and progestrone on chemical epliepsy in rats. Material and Methods: This experimental study was performed on 60 male rats and randomizely divided in to six group (one control group and five experimental groups). In all groups, rats kindeled by pentylene tetrazol (PTZ) kindling model. In this modle rats recieved PTZ (50 mg/kg) ip once at 48 hours for 12 sessions. In experimental groups, Rats recieved vehicle or progestrone (25, 50 mg/kg) or stradiol valorate (5, 10mg/kg) 20 min before PTZ injection and serizure stages were measured for 20 min. Results: Our results indicated that, PTZ injection increased the average of seizure stage and in final injection, 71.43% of rats shows the five stage of seisure. Stradiol don’t significant effect on seizure stages but it clinically increased the mortality rate (P<0.05). whreas, progestrone significantly decreased the seizure stages development (P<0.01) and mortality (P<0.05). Conclusion: finally, we conclude that progestrone attenuate the seizure stages, development stradiol increased the mortality rate during seizure.

Keywords: Estradiol, Progestrone, Epilepsy, PTZ, Kindling