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Journal of Research in Medical Sciences، جلد ۱۶، شماره ۱۱، صفحات ۰-۰
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عنوان انگلیسی Entamoeba histolytica and Entamoeba dispar prevalence among patients with gastrointestinal complaints in Chelgerd city, southwest of Iran
چکیده انگلیسی مقاله Normal 0 false false false EN-US X-NONE FA BACKGROUND : Differentiation between Entamoeba histolytica and Entamoeba dispar is very important for both clinical therapy and epidemiological studies. Although these two species are morphologically identical, they have differences in genetic, chemical specifications and pathogenicity. This study was carried out to differentiate E. histolytica from E. dispar and also to find out prevalence of the two species. METHODS : Fecal samples were collected three times from 655 patients with gastrointestinal complaints (47.3% male), who were referred to the primary health care centers of Chelgerd, Chaharmahal and Bakhtiary province. Samples were examined microscopically with direct smear, formalin-ethyl-acetate concentration and trichrom staining methods to distinguish E. histolytica from E. dispar complex and differentiate them from non-pathogenic intestinal amoeba. Genomic DNA was extracted from microscopy positive isolates and polymerase chain reaction (PCR) was carried out to different the two morphologically identical Entamoeba isolates. RESULTS : Among the 655 recruited patients, eleven subjects with E. histolytica / E. dispar isolates (1.7%) were identified by microscopy methods. Ten of the positive isolates (90.9%) were identified as E. histolytica by PCR and one isolate (9.09 %) was positive for E. dispar. CONCLUSIONS : This study revealed that E. histolytica was more prevalent than E. dispar in the studied area. This result was different from the previously reported data in other parts of Iran. KEYWOEDS : Gastrointestinal Complaints, Entamoeba Histolytica, Entamoeba Dispar, Polymerase Chain Reaction, Iran .
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نویسندگان مقاله نادر پسته چیان | nader pestehchian
assistant professor, department of medical parasitology and mycology, school of medicine, isfahan university of medical sciences, isfahan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

مهناز نظری | mahnaz nazary
shahrekord health center, sharekord university of medical sciences, sharekord, iran


علی حقیقی | ali haghighi
professor, department of medical parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

منصور صالحی | mansour salehi
associate professor, department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

حسینعلی یوسفی | hosinali yosefi
instructor, department of medical parasitology and mycology, school of medicine, isfahan university of medical sciences, isfahan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

نشانی اینترنتی http://jrms.mui.ac.ir/index.php/jrms/article/view/6896
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Articles
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