این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Research in Medical Sciences، جلد ۷، شماره ۲، صفحات ۰-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی DETECTION AND ISOLATION OF CD59 FROM HUMAN SEMINAL PLASMA
چکیده انگلیسی مقاله Introduction. CD59 is one of the complement regulatory proteins (CRPs) which exert inhibitory function by blocking the formation of C5b-9 complex or Membrane Attacks complex (MAC). Regarding the therapeutic role of CD59 in treatment of pathological effects in uncontrolled activation of complements system and its efficiency to overcome the hyper-acute rejection, CD59 was suggested for maintenance of transplanted organ. In this study We determined and isolated CD59 from seminal plasma. Methods. Six normospermic sample according to WHO standards were chosen. Plasma of samples was separated and to remove the postasomes, the seminal plama was ultra centrifuged. CD59 was detected by Dot-Blot using CD59 mAb (MEM43). The molecular weight and purity of protein was detected by SOS-PAGE method follwed by Westerm Blot. Results. Protein was present in the 6.5 ml and 15ml of gel fitration fractions. Molecular weight based on marker size in these two fractions was 65 and 21KD respectively. Discussion. CD59 had previously beem purified by lysis of erythrocyte cell membrane. Because of use of detergent and preservative agents, this method decreased physiologic effects of the protein. In this study the isolation was performed from prostasome granules" without using of any detergent and preservative agents.
کلیدواژه‌های انگلیسی مقاله C059, Complement Regulatory Protein, Seminal Plasma
نویسندگان مقاله عبدالمجید رضایی | a rezaie
department of immunology, school of medicine, isfahan university of medical sciences and health services, isfahan,iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

m h نصر اصفهانی | m h nasr esfahani


محمود هاشمی تبار | mahmoud hashemi tabar


h بهرامیان | h bahramian


f عریضی | f oraizi


نشانی اینترنتی http://jrms.mui.ac.ir/index.php/jrms/article/view/3249
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات