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International Journal of Organ Transplantation Medicine، جلد ۴، شماره ۳، صفحات ۰-۰
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عنوان انگلیسی Isolation and Characterization of Human Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton’s Jelly and Amniotic Membrane
چکیده انگلیسی مقاله Background: Mesenchymal stem cells (MSCs) have a capacity for self-renewal and multi-potential differentiations. These cells are considered powerful sources for cell therapy in regenerative medicine and tissue engineering. The cells can be isolated from various tissues; however, harvesting from human umbilical cord and amniotic membrane is easy and accessible source. Objective: To isolate and characterize the MSCs derived from human umbilical cord Wharton’s jelly (WJMSC) and amniotic membrane (AM-MSC) with regard to their morphology, immunophenotype and mesodermal differentiation potential in order to obtain an alternative source of MSC for therapeutic clinical applications. Methods: Fetal membranes and umbilical cords (n=3) were retrieved from healthy full-term women by elective cesarean delivery. Amniotic membrane and umbilical cord were separately minced and cultured in DMEM supplemented with 10% FBS. After reaching 80% of confluency, the umbilical cord WJ-MSC and AM-MSC were characterized by expression of cell surface markers with flowcytometry, stem cell gene expression with adipogenic/osteogenic potential. Results: Both WJ-MSC and AM-MSC were spindle-shaped cells, expressed MSC surface markers in flowcytometry and stem cell transcriptional factors (OCT4 and NANOG). After induction, the cells differentiated into adipogenic and osteogenic lineages. Conclusion: MSC were successfully generated from umbilical cord WJ-MSC and AM-MSC with similar mesenchymal markers and properties.
کلیدواژه‌های انگلیسی مقاله Mesenchymal Stromal cells,Amnion,Umbilical cord
نویسندگان مقاله t پیرجلی | t pirjali


n آذرپیرا | n azarpira


m آیت اللهی | m ayatollahi


mh aghdai | mh aghdai


b گرامی زاده | b geramizadeh


t طلایی | t talai


نشانی اینترنتی http://www.ijotm.com/ojs/index.php/IJOTM/article/view/138
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Articles
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