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International Journal of Organ Transplantation Medicine، جلد ۳، شماره ۱، صفحات ۰-۰
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عنوان انگلیسی Human Bone Marrow-derived Mesenchymal Stem Cell: A Source for Cell-Based Therapy
چکیده انگلیسی مقاله Background: The ability of mesenchymal stem cells (MSCs) to differentiate into many cell types, and modulate immune responses, makes them an attractive therapeutic tool for cell transplantation and tissue engineering. Objective: This project was designed for isolation, culture, and characterization of human marrow-derived MSCs based on the immunophenotypic markers and the differentiation potential. Methods: Bone marrow of healthy donors was aspirated from the iliac crest. Mononuclear cells were layered over the Ficoll-Paque density-gradient and plated in tissue cultures dish. The adherent cells expanded rapidly and maintained with periodic passages until a relatively homogeneous population was established. The identification of adherent cells and the immune-surface markers was performed by flow cytometric analysis at the third passage. The in vitro differentiation of MSCs into osteoblast and adipocytes was also achieved. Results: The MSCs were CD11b (CR3), CD45, CD34, CD31 (PCAM-1), CD40, CD80 (B7-1), and HLA-class II negative because antigen expression was less than 5%, while they showed a high expression of CD90, and CD73. The differentiation of osteoblasts, is determined by deposition of a mineralized extracellular matrix in the culture plates that can be detected with Alizarin Red. Adipocytes were easily identified by their morphology and staining with Oil Red. Conclusion: MSCs can be isolated and expanded from most healthy donors, providing for a source of cell-based therapy.
کلیدواژه‌های انگلیسی مقاله Mesenchymal stem cells,Human bone marrow,cell therapy
نویسندگان مقاله m آیت اللهی | m ayatollahi


b گرامی زاده | b geramizadeh


m ذاکری نیا | m zakerinia


m رمزی | m ramzi


r یعقوبی | r yaghobi


p حدادی | p hadadi


ar رضوانی | ar rezvani


m aghdai | m aghdai


n آذرپیرا | n azarpira


h کریمی | h karimi


نشانی اینترنتی http://www.ijotm.com/ojs/index.php/IJOTM/article/view/94
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Articles
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