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Cell Journal، جلد ۱۹، شماره ۳، صفحات ۴۰۳-۴۱۴
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عنوان انگلیسی Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: The diverse clinical applications for human mesanchymal stem cell (hMSCs) in cellular therapy and regenerative medicine warrant an increased focus on developing adequate culture supplement devoid of animal-derived products. In the present study, we have investigated the feasibility of umbilical cord blood Platelet Lysate (UCB-PL) as a standard substitute for fetal bovine serum (FBS) and human peripheral blood Platelet Lysate (PB-PL). Materials and methods: In this experimental study, platelet concentrates (PC) from UCB and human PB donors were frozen, melted and sterilized to obtain PL. Quality control included platelet cell counts, sterility testing (viral and microbial), total protein concentrations, growth factor levels, and PL stability. The effects of UCB-PL and PB-PL on hMSCs proliferation and differentiation into osteocytes, chondrocytes and adipocytes were studied and the results compared with FBS. Results: Our results determined that UCB-PL contained high levels of protein content, platelet-derived growth factor -AB (PDGF-AB), and transforming growth factor (TGF) compared to PB-PL. All growth factors were stable for at least nine months post-storage at -70ºC. MSCs proliferation enhanced following treatment with UCB-PL. With all three supplements, MSCs could differentiate into all three lineages. Conclusion: According to our results, PB-PL and UCB-PL both were potent in hMSCs proliferation. However, PB promoted osteoblastic differentiation and UCB-PL induced chondrogenic differentiation. Because of availability, easy to use and fesibel standardization of UCB-PL, , we suggested that UCB-PL as an alternative to FBS and PB-PL for the cultivation and expansion of hMSCs in cellular therapy.
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نویسندگان مقاله | Negin Shirzad


| Sima Bordbar


| Alireza Goodarzi


| Monire Mohammad


| Pardis Khosravani


| Froughazam Sayahpour


| Mohamadreza Baghban Eslaminejad


| Marzieh Ebrahimi


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4886
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