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Cell Journal، جلد ۱۹، شماره ۳، صفحات ۴۱۵-۴۲۴
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عنوان انگلیسی Caffeine Augments The Instruction of Anti-Inflammatory Macrophages by The Conditioned Medium of Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: It has been revealed that mesenchymal stem cells (MSCs) produce adenosine and express adenosine receptors and communicate with other cells like macrophages. However, there is no information about the role of caffeine, as a popular environmental factor and adenosine antagonist, on the crosstalk between MSCs and immune cells. The aim of the current study was to evaluate the effects of the conditioned medium of MSCs treated with caffeine on macrophages. Materials and methods: MSCs were isolated from bone marrow of rats and pulsed with different concentrations of caffeine (0, 0.1, 0.5 and 1 mM) for 72 h. The conditioned medium of MSCs was collected after 24 h, and incubated with macrophage for 24 h. Finally, the functions of macrophages were evaluated. Results: The data showed that the conditioned medium of MSCs treated with caffeine significantly enhanced the phagocytosis and simultaneously regressed the expression of the reactive oxygen substances and nitric oxide as well as IL-12 by macrophages more profound than supernatants of MSCs alone. Moreover, the conditioned medium of MSCs pulsed with caffeine at low to moderate concentrations preserved the neutral red uptake by macrophages and elevated the IL-10 secretion by macrophages. A high concentration of caffeine could interfere with two latter effects of supernatants of MSCs on the macrophages. Conclusion: Collectively, it seems that caffeine treatment of mesenchymal stem cells augments the instruction of anti-inflammatory macrophage by conditioned medium mesenchyme stem cells. These findings may offer new insight into the potential mechanisms underlying the immunomodulatory and anti-inflammatory effects of caffeine.
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نویسندگان مقاله | Nazanin Shushtari


| Seyyed Meysam Abtahi Froushani


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4364
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