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Cell Journal، جلد ۱۹، شماره ۳، صفحات ۴۳۴-۴۴۲
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عنوان انگلیسی Assessment of The Dose-Response Relationship of Radiation-Induced Bystander Effect in Two Cell Lines Exposed to High Doses of Ionizing Radiation (6 and 8 Gy)
چکیده انگلیسی مقاله Objective: Dose-response relationship of radiation-induced bystander effect (RIBE) is somehow controversial at high dose levels. The aim of the present study was to assess RIBE at high doses by examination of different endpoints. Materials and methods: Medium transfer technique was performed to induce bystander effect. Two main groups, including QU-DB cells, which received medium from autologous irradiated cells and MRC5 cells, which received medium from irradiated QU-DB cells, were examined. Colony, MTT and, Micronucleus assays were performed to quantify bystander responses. Also, the medium was diluted and transferred to bystander cells, to investigate whether medium dilution can revive RIBE response which was disappeared at a high dose? Results: RIBE level in QU-DB bystander cells increased in the dose range of 0.5 to 4 Gy, but it decreased at 6 and 8 Gy surprisingly. MN frequency of QU-DB bystander cells which received the most diluted medium from 6 and 8 Gy QU-DB irradiated cells, reach to the maximum levelthan MN frequency of cells that received complete medium P < 0.0001 and MN frequency of MRC5 cells which received the most diluted medium from 4Gy QU-DB irradiated cells reach to the maximum levelthan MN frequency of cells that received complete medium P < 0.0001. Conclusion: Our results showed, RIBE level decreased at doses above 4 Gy; however, it was increased when the conditioned medium transferred to bystander cells was diluted. It confirmed that a negative feedback mechanism is responsible for decreasing the RIBE response at high doses. Decrease of RIBE at high doses of ionizing radiation may be applicable to predict normal tissue damage as a result of RIBE in radiotherapy modalities such as radiosurgery, brachytherapy and gridtherapy which apply high dose fractionation.
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نویسندگان مقاله | Mohammad Taghi Bahreyni Toossi


| Sara Khademi


| Hosein Azimian


| Shokoufeh Mohebbi


| Shokouhozaman Soleymanifard


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4343
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