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Cell Journal، جلد ۱۹، شماره ۳، صفحات ۴۷۶-۴۸۱
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عنوان انگلیسی Effect of Fetal Mouse Lung Tissue Co-Culture on In Vitro Maturation of Mouse Immature Oocytes
چکیده انگلیسی مقاله Background: The aim of this study was to evaluate fetal Mouse lung tissue co-culture on in vitro maturation of mouse immature oocytes. Materials and methods: GV oocytes as immature oocytes from ovaries of about 25 , 6-8 weeks female mice were dissected after being stimulated by an injection of 7.5 IU pregnant mare serum gonadotropin (PMSG) intra peritoneal . The fetal lung tissues were prepared from 10-14 day old embryos of 15 pregnant mice and were cultured individually in (α-MEM) supplemented with 10% (FBS), 100 μg/ml penicillin and 50 μg/ml streptomycin as base medium. A total number of 300 oocyte were cultured in three groups for 24 houres, 1- Base medium as control group (n=100) 2, 3 experimental groups contains base medium co-cultured with 11.5 to 12.5 day old and 12.5 to 13.5 day old fetal mouse lung tissues respectively (n=100) for each group. The proportion of GV oocytes matured into metaphase ІІ oocytes in the three groups were compared with ANOVA test. Correlation test were used for evaluation of oocyte in vitro maturation rate. Results: The proportions of GV oocytes matured into metaphase ІІ oocytes were 46%, 65%, 56%, in control group and experimental groups 2,3 respectively (P < 0.05). The percentage of the oocytes remained at the GV stage was higher in control group than the other groups (P < 0.05). Conclusion: This study suggests that co-culture with fetal Mouse lung tissue increase the percentage of germinal vesicle in to Metaphase II oocyte.
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نویسندگان مقاله | Masomeh Belbasi


| Seyed Gholam Ali Jorsaraei


| Maryam Gholamitabar Tabari


| Ramzan Khanbabaei


نشانی اینترنتی http://celljournal.org/journal/article/abstract/3866
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