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Cell Journal، جلد ۱۹، شماره ۳، صفحات ۴۸۲-۴۹۱
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عنوان انگلیسی Evidence of Oocyte Polarity in Bovine; Implications for Intracytoplasmic Sperm Injection and Somatic Cell Nuclear Transfer
چکیده انگلیسی مقاله Objective: In lower vertebrates, the spatial restriction of molecules and cues within the animal and vegetal poles of oocyte is a well-documented fact with important consequences on the progression and proper outcome of embryonic development. However, whether the same form of oocyte polarity also exists in mammals is currently under hot debate. Here, we investigated the likelihood of oocyte polarity for the first time in bovine. Materials and methods: In vitro matured bovine oocytes were used for manual bisection [into oocyte halve that were near-to (HNS) and far-from (FS) spindle] or trisection [into MII-spindle (S), the spindle-side half (NS), and the distal half unassociated with the spindle (FS)]. Prepared pools of oocyte substructures were used for comparative RT-qPCR. To map the possible preferential sperm entry point (SEP), the spatial relationship between SEP and MII-spindle was measured 5 hours post-fertilization. Results: The proportional amount of maternal mRNA in Soocyte fragment was estimated to be 6 to 11 -fold higher than NS and FS counterparts. The relative abundances of NANOG, OCT4, FGF4, and TEAD4 were significantly higher in HNS oocyte fragment compared t0 FS. The relative abundances of CTNB, CARM1, REX1, SOX2, and CDX2 were comparable between HNS and NS oocyte fragments. FS oocyte fragment possessed significantly higher transcripts of GATA4 compared to HNS. The distribution of certain transcripts related to pluripotency and lineage commitment were different depending upon the region of the oocyte; either enriched at S (TEAD4, NANOG, CTNB and SOX2), NS (OCT4), or FS (GATA6). The SEP in almost (90%) fertilized oocytes was located in MII-hemisphere. Conclusion: The observation of spatial restriction of mRNAs and SEP within MII-oocyte may indicate that the principal forces of oocyte polarity are evolutionary conserved. This may in turn highlight the need for refinements in the methodology of intracytoplasmic sperm injection (where a sperm is injected far from the MII-spindle) and somatic cell nuclear transfer (where a huge amount of regulative mRNAs that are associated with MII-spindle is removed during enucleation).
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نویسندگان مقاله | Seyed Morteza Hosseini


| Fariba Moulavi


| Nima TanhaieVash


| Naser Shams-Esfandabadi


| Mohammad Hossein Nasr-Esfahani


| Abolfazl Shirazi


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4887
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