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Cell Journal، جلد ۱۹، شماره ۳، صفحات ۵۱۲-۵۱۹
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عنوان انگلیسی Taraxerol Induces Cell Apoptosis through A Mitochondria-Mediated Pathway in HeLa Cells
چکیده انگلیسی مقاله Objective: Taraxerol acetate induced potent anti-cancer effects via the induction of apoptosis, autophagy, cell cycle arrest and inhibition of cell migration. However, whether taraxerol induced apoptosis and its underlying mechanisms of action is not clear. In the present study, we assessed taraxerol on mitochondrial apoptotic pathway effect, and determined the release of cytochrome c to the cytosol and the activation of caspases. Materials and methods: We mainly investigated the effect of taraxerol on HeLa cells. We tested cell viability by MTT assay and morphologic changes, analyzed apoptosis by DAPI staining and flow cytometer. We also determined ROS and mitochondrial membrane potential using Microplate Reader. In addition, the apoptotic proteins were tested by Western blot. Results: Taraxerol enhanced ROS levels and attenuated the mitochondrial membrane potential (Δψm) in HeLa cells. Furthermore, taraxerol induced apoptosis mainly via the mitochondrial pathway including the release of cytochrome c to the cytosol and the activation of caspases 9, caspases 3 and PARP. Taraxerol could induce the down-regulation of anti-apoptotic proteins Bcl-2 and up-regulation of pro-apoptotic proteins Bax levels. It suppressed PI3K/Akt signaling pathway. Conclusion: These results demonstrated taraxerol induces cell apoptosis through mitochondria-mediated pathway in HeLa cells. Thus, taraxerol may be a potential anti-cervical cancer candidate.
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نویسندگان مقاله | Xiangyang Yao


| Binyu Lu


| Chaotian Lü


| Qin Bai


| Dazhong Yan


| Hui Xu


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4543
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