این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۲۱، شماره ۱، صفحات ۲۷-۳۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Amentoflavone Induces Autophagy and Modulates p53
چکیده انگلیسی مقاله Objective: Amentoflavone is a main component of Selaginella tamariscina widely known as an oriental traditional medicinal stuff that has been known to have a variety of medicinal effects such as the induction of apoptosis, anti-metastasis and anti-inflammation. However, the effect of amentoflavone on autophagy has not been reported until now. The aim of this study was to investigate whether amentoflavone has a positive effect on the induction of autophagy related to cell aging. Materials and methods: The aging of young cells was induced by treatment with IGF-1 at 50 ng/mL three times every two days. The effect of amentoflavone on cell viability was evaluated in A549 and WI-38 cells using MTT assay. The induction of autophagy was detected using autophagy detection kit. The expression of proteins related to autophagy and IGF-1 signaling pathway was examined by western blot analysis and immunofluorescence assay. Results: First of all, it was found that amentoflavone induces formation of autophagosome. In addition, it enhanced the expression level of Atg7 and increased the expression levels of Beclin1, Atg3 and LC3 associated with induction of autophagy in immunofluorescence staining and western blot analyses. Moreover, amentoflavone inhibited cell aging induced by IGF-1 and hydrogen peroxide. In particular, the levels of p53 and p-p21 were increased in the presence of amentoflavone. Furthermore, amentoflavone increased the level of SIRT1 deacetylating p53. Conclusion: Our results suggest that amentoflavone could play a positive role in the inhibition of various diseases associated with autophagy and modulation of p53.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Hye-Jung Park


| Moon-Moo Kim


نشانی اینترنتی http://celljournal.org/journal/article/abstract/5717
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-1043610.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات