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Cell Journal، جلد ۲۱، شماره ۱، صفحات ۵۷-۶۱
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عنوان انگلیسی Induction Effects of Bacteroides fragilis Derived Outer Membrane Vesicles on Toll Like Receptor 2, Toll Like Receptor 4 Genes Expression and Cytokines Concentration in Human Intestinal Epithelial Cells
چکیده انگلیسی مقاله Abstract Objective: The gastrointestinal tract like other mucosal surface is colonized with a microbial population known as gut microbiota. Outer membrane vesicles (OMVs) which are produced by Gram negative bacteria could be sensed by Toll like receptors (TLRs). The interaction between gut microbiota and TLRs affects homeostasis and immune responses. In this study, we evaluated TLR2, TLR4 genes expression and cytokines concentration in Caco-2 cell line treated with Bacteroides fragilis and its OMVs. Materials and Methods: In this experimental study, OMVs were extracted using sequential centrifugation and their physicochemical properties were evaluated as part of quality control assessment. Caco-2 cell line was treated with B. fragilis and its OMVs (180 and 350 µg/ml). Quantitative PCR (qPCR) was performed to quantify TLR2 and TLR4 mRNA expression. Pro- inflammatory (IFNᵧ) and anti- inflammatory (IL-4 and IL-10) cytokines were assessed by ELISA. Results: B. fragilis significantly decreased TLR2 and slightly increased TLR4 mRNA levels in Caco-2 cell line. The TLR2 mRNA level did not change at 180 and 350 µg/ml of OMVs. Conversely, the TLR4 mRNA level significantly was decreased and increased at 180 and 350 µg/ml of OMVs respectively. Furthermore, B. fragilis and its OMVs significantly increased and decreased IFNᵧ concentration respectively. Anti-inflammatory cytokines were increased by B. fragilis and its OMVs. Conclusion: B. fragilis and its OMVs have pivotal role in the cross talk between gut microbiota and the host especially in the modulation of the immune system. Based on last studies on immunomodulatory effect of B. fragilis derived OMVs on immune cells and our results, we postulate that B. fragilis derived OMVs could be possible candidate for the reduction of immune responses.
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نویسندگان مقاله | Sara Ahmadi Badi


| Shohreh Khatami


| Shiva Irani


| Seyed Davar Siadat


نشانی اینترنتی http://celljournal.org/journal/article/abstract/5750
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