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Avicenna Journal of Phytomedicine، جلد ۶، شماره ۶، صفحات ۶۷۱-۶۷۷
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Effects of Biebersteinia multifida hydro-ethanol extract on proliferation and apoptosis of human prostate cancer and human embryonic kidney cells
چکیده انگلیسی مقاله Objective: Biebersteinia (Geraniaceae) has a history of use in traditional medicine in some countries including Iran. In the present study, cytotoxic and apoptogenic properties of hydro-ethanol extract of B. multifidi was investigated on human prostate cancer cell lines (PC3 and DU 145) and human embryonic kidney 293 (HEK293) cells. Materials and Methods: Cells were cultured in RPMI-1640 medium supplemented with 10% FBS at 37ºC in a humidified atmosphere of 95% air and 5% CO2. The root of the plant was macerated with EtOH 70%. Cytotoxic activity of ethanol extract of B. multifida was assessed using alamarBlue® assay after 48 hr of treatment. Apoptotic cells were stained with propidium iodide (PI) and detected by flow cytometry (sub-G1 peak). Results: B. multifidi had cytotoxic effect on malignant cells and normal HEK293 cells in a dose-dependent manner and significantly decreased the cell viability (IC50 values were between 199.2 and 302.9 µg/ml). B. multifida increased the sub-G1 peak in flow cytometry histogram of treated PC3 cells compared to control showing the induction of apoptosis and DNA fragmentation. Conclusion: Due to cytotoxic and apoptotic activity of B. multifida, the plant is suggested for further phytochemical analysis and mechanistic evaluation.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله علیرضا گلشن | alireza golshan
medicinal plants amp;amp; natural products research center mpnprc , north khorasan university of medical sciences, bojnurd, iran


سمیرا حسن زاده | samira hassanzadeh
pharmacological research centre of medicinal plants, school of medicine, mashhad, university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

مریم مژد کانلو | maryam mojdekanloo
north khorasan university of medical sciences, bojnurd, iran


زهرا طیرانی نجاران | zahra tayarani najjaran
pharmacological research centre of medicinal plants, school of medicine, mashhad, university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ajp.mums.ac.ir/article_6793.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Research Article
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