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Avicenna Journal of Phytomedicine، جلد ۶، شماره ۵، صفحات ۴۸۹-۴۹۴
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عنوان انگلیسی Capparis spinosa reduces Doxorubicin-induced cardio-toxicity in cardiomyoblast cells
چکیده انگلیسی مقاله Objective: Doxorubicin (DOX) is an effective anticancer drug but its clinical application is limited because it induces apoptosis in cardiomyocytes and leads to permanent degenerative cardiomyopathy and heart failure possibly due to oxidative stress. Recent studies showed that Capparis spinosa (C. spinose)exhibits potent antioxidant activity. So, in this study, we explored the protective effect of hydro-alcoholic extract of C. spinosa against DOX-induced cytotoxicity in H9c2 cells. Materials and Methods: Cell viability was quantified by MTT assay. Apoptotic cells were determined using flow cytometry (sub-G1 peak) evaluation of DNA fragmentation following PI staining. Cells were cultured with 5 μM DOX for 24 hr to induce cell damage. H9c2 cells were pretreated with different concentrations (6-200 μg/ml) of C. spinosa extract for 4 hr before DOX treatment in all trials. Results: Pretreatment with 25, 50, 100 and 200 µg/ml of C. spinosa could increase the viability of H9C2 cells to 72.63 ± 2.8% (p< 0.05), 77.37 ± 1.8% (p< 0.05), 83.56 ± 2.6% (p< 0.001) and 90.9 ± 0.5% (p< 0.001) of control, respectively. Also, C. spinosa decreased apoptotic induction significantly, at the doses of 50 µg/ml (p< 0.05), 100 µg/ml (p< 0.01) and 200 µg/ml (p< 0.001) Conclusion: Our results showed that C. spinosa could exert cardioprotective effects against DOX-induced toxicity that might be mediated via its antioxidant activity.
کلیدواژه‌های انگلیسی مقاله Capparis spinosa, H9C2 cells, Doxorubicin, Apoptosis
نویسندگان مقاله سید هادی موسوی | seyed hadi mousavi
pharmacological research center of medicinal plants, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

آذر حسینی | azar hosseini
pharmacological research center of medicinal plants, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

الهام بختیاری | elham bakhtiari
department of pharmacology, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

حسن رخشنده | hassan rakhshandeh
department of pharmacology, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ajp.mums.ac.ir/article_6207.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Short communication
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