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Avicenna Journal of Phytomedicine، جلد ۶، شماره ۴، صفحات ۴۳۴-۴۴۱
عنوان فارسی
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عنوان انگلیسی Protective effect of Viola tricolor and Viola odorata extracts on serum/glucose deprivation-induced neurotoxicity: role of reactive oxygen species
چکیده انگلیسی مقاله Objective: Oxidative stress plays a key role in the pathophysiology of brain ischemia and neurodegenerative disorders.Previous studies indicated that Viola tricolor and Viola odorataare rich sources of antioxidants. This study aimed to determine whether these plants protect neurons against serum/glucose deprivation (SGD)-induced cell death in an in vitro model of ischemia and neurodegeneration. Methods and Material: The PC12 neuronal cells were pretreated for 4 hr with 1 to 50 µg/ml of V. odorata or V. tricolor hydroalcoholic extracts followed by 24 hr incubation under SGD condition. Cell viability was evaluated by 4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2H-tetrazolium bromide assay. The level of intracellular reactive oxygen species (ROS) was quantitated by flow cytometry using 2',7'- dichlorofluorescin diacetate as a probe. Results: SGD condition led to significant decrease in cell viability (p < 0.001). Pretreatment with both V. tricolor and V. odorata extracts reduced the SGD-induced cytotoxicity. SGD resulted in a significant increase in intracellular ROS production (p < 0.001). Both extracts at concentrations of 25 and 50 µg/ml could reverse the increased ROS production (p < 0.05). Conclusion: Results of the present study showed that V. tricolor and V. odorata protect neuronal cells against SGD-induced cell death, at least in part, by their antioxidant activities. Further studies on the possible application of these plants in prevention or treatment of cerebral ischemia and neurodegenerative diseases seem to be warranted.
کلیدواژه‌های انگلیسی مقاله PC12, Reactive oxygen species, Viola tricolor, Viola odorata
نویسندگان مقاله سید هادی موسوی | seyed hadi mousavi
department of pharmacology, faculty of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

بهناز نقی زاده | behnaz naghizade
payam noor shargh, tehran university of basic sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

سولماز pourgonabadi | solmaz pourgonabadi
department of pharmacology school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

احمد قربانی | ahmad ghorbani
pharmacological research center of medicinal plants, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ajp.mums.ac.ir/article_6327.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Research Article
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