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Basic and Clinical Neuroscience، جلد ۹، شماره ۶، صفحات ۵-۶
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عنوان انگلیسی Different Expressions of Specific Transcription Factors of Th1 (T-bet) and Th2 cells (GATA-3) by Peripheral Blood Mononuclear Cells From Patients With Multiple Sclerosis
چکیده انگلیسی مقاله Introduction: Multiple Sclerosis (MS) is an inflammatory disorder caused by self-reactive Th1 lymphocytes, while Th2 cells may confer protection. The Th1 and Th2 cell differentiation are regulated by specific transcription factors, especially T-bet and GATA-3, respectively. This investigation aimed to measure the T-bet and GATA-3 expression by Peripheral Blood Mononuclear Cells (PBMCs) obtained from MS patients after specific and non-specific in vitro stimulation. Methods: The PBMCs were separated from 22 patients with MS and 20 healthy individuals. They were cultured at 37°C for 24 h in the absence of a stimulator or in the presence of Myelin oligodendrocyte Glycoprotein (MOG) or Phytohemagglutinin (PHA) at a concentration of 10 μg/mL. Then the T-bet and GATA-3 expression was measured by real time-PCR. Results: The T-bet expression was enhanced, while the GATA-3 expression diminished. Therefore the expression of T-bet/GATA-3 ratio diminished in not-stimulated, MOG-stimulated and PHA-stimulated PBMCs from MS patients compared with equal cultures from the healthy individuals (P< 0.01, P< 0.01 and P< 0.01, for T-bet; P< 0.03, P< 0.01 and P< 0.02, for GATA-3; P< 0.01, P< 0.001 and P< 0.01 for T-bet/GATA-3 ratio, respectively). The not-stimulated, MOG-stimulated, and PHA-stimulated PBMCs from men with MS expressed higher amounts of GATA-3 than equal cells from MS women (P< 0.05, P< 0.05 and P< 0.01, respectively). Conclusion: These results probably indicate an imbalance in Th1/Th2 cells in the level of transcription factors with a tendency toward Th1 cells in MS. The clinical utilization of the transcription factors as novel biomarkers of MS should be evaluated in further studies.
کلیدواژه‌های انگلیسی مقاله Multiple Sclerosis, Th1, Th2, T-bet, GATA-3
نویسندگان مقاله | Zahra Etesam
Neuroscience Research Center, Department of Neurology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.


| Maryam Nemati
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.


| Mohammad-Amin Ebrahimizadeh
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.


| Hossain-Ali Ebrahimi
Neuroscience Research Center, Department of Neurology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.


| Hossain Hajghani
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.


| Tahereh Khalili
Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.


| Abdollah Jafarzadeh
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
نشانی اینترنتی http://bcn.iums.ac.ir/browse.php?a_code=A-10-900-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cellular and molecular Neuroscience
نوع مقاله منتشر شده Original
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