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Iranian Journal of Basic Medical Sciences، جلد ۲۲، شماره ۱، صفحات ۱۰۶-۱۱۱
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عنوان انگلیسی Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China
چکیده انگلیسی مقاله Objective(s): Acinetobacter pittii has become an emerging opportunistic noscomial pathogen worldwide with multi-drug resistance. In the present study, an A. pittii strain was isolated from bronchoalveolar lavage fluid sample harboring both OXA-58 and NDM-1carbapenemase producing genes. The mechanisms of carbapenem resistance of the A. pittii strain was investigated.Materials and Methods: Carbapenemase producing genes were examined by PCR and DNA sequencing. S1-PFGE was used to localize carbapenemase encoding genes. Filter mating and electrotransformation were used to investigate the transferability of such carbapenemase encoding genes between different strains. Genetic surroundings of blaOXA-58 and blaNDM-1 genes were detected as well.Results: The A. pittii strain, carrying both OXA-58 and NDM-1 carbapenemase encoding genes, was resistant to all β-lactam antibiotics, while suscepitible to ciprofloxacin, levofloxacin, tobramycin, cotrimoxazole and tigecycline. Southern blot hybridization for the blaOXA-58 and blaNDM-1 gene indicated that the two genes locate in the same plasmid with molecular weight of 310.1-336.5kb. BlaOXA-58 was located in an ISAba3-blaOXA-58-ISAba3-like structure, and the blaNDM-1 gene cluster was embedded in an ISAba125-aphA6- blaNDM-1-bleMBL-ΔtrpF-dsbC-cutA structure sequentially.Conclusion: In the present study, it is first reported an A. pittii clincal strain in China, co-harboring OXA-58 and NDM-1 carbapenemase producing genes residing on a same plasmid. In hospital and community settings, it is of great significance and urgence to increase the surveillance of these kinds of organisms.
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نویسندگان مقاله | Yili Chen
Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China


| Penghao Guo
Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China


| Han Huang
Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China


| Yongxin Huang
Department of Laboratory Medicine, Zhongshan Medical School of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China


| Zhongwen Wu
Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China


| Kang Liao
Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China
نشانی اینترنتی http://ijbms.mums.ac.ir/article_11601.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Short Communication
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