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Research in Pharmaceutical Sciences، جلد ۱۳، شماره ۶، صفحات ۵۵۷-۵۶۵
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عنوان انگلیسی Galectin-9 induces apoptosis in OVCAR-3 ovarian cancer cell through mitochondrial pathway
چکیده انگلیسی مقاله Galectin-9 (Gal-9), a member of animal lectins’ family, is implicated in the induction of apoptosis in various cancer cells. Here, we evaluated the anti-tumor effect of Gal-9 in OVCAR-3 ovarian cancer cells. The effect of the Gal-9 on cell viability was evaluated using MTT assays. Apoptosis was assessed using Annexin-V staining. The assessment of mitochondrial membrane potential (ΔΨm) was performed using a JC-1 probe. The activity of caspase-3 and caspase-6 were evaluated with colorimetric assay. The production of reactive oxygen species (ROS) was applied by fluorescent probe. The expression levels of Bax and Bcl-2 were assessed using western blotting. The result showed that Gal-9 inhibits cell viability. Flow cytometry analysis showed that Gal-9 induces apoptosis in ovarian cancer cells. Moreover, Gal-9 decreased ΔΨm and increased the generation of ROS and caspase-3 and caspase-6 activities in ovarian cancer cells. Moreover, Gal-9 induced expression of Bax as well as inhibited expression of Bcl-2. In conclusion, our results indicated that Gal-9 induced apoptosis in ovarian cancer cells through mitochondrial pathway.
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نویسندگان مقاله | Seyyed Mehdi Jafari
3Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Ali Nazri
4Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran.


| Mahdi Shabani
3Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Narges Zargar Balajam
3Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. 5Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Mahmoud Aghaei


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/1868
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/115/article-115-1075802.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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