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Cell Journal، جلد ۱۹، شماره ۱، صفحات ۵۵-۶۵
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عنوان انگلیسی A novel approach on drug delivery: Investigation of new nano-formulation of liposomal doxorubicin and biological evaluation of entrapped doxorubicin on various osteosarcomas cell lines
چکیده انگلیسی مقاله Objective: In this study, a novel formulation of liposomal Doxorubicin (DOX) was prepared. The drug dose was optimized by analyzing cellular uptake and cell viability of osteosarcoma (OS) cell lines upon exposure to the nanoliposomes containing varying DOX concentrations, with the goal to reduce cytotoxicity of DOX and improve the characteristics of the Nano Systems. Materials and methods: In this experimental study, liposomes were prepared using the pH gradient hydration method. Various characterization tests (such as dynamic light scattering (DLS), cryogenic transmission electron microscopy (TEM) imaging, and UV-Vis spectrophotometry) were employed to evaluate the quality of the nanocarriers. In addition, the CyQuant assay and fluorescence microscope imaging were used on various OS cell lines (MG-63, U2-OS, SaOS-2, SaOS-LM7) and primary human bone cells, as novel methods to determine cell viability and in vitro transfection efficacy, respectively. Results: The entrapment efficiency of DOX within the optimized liposomal formulation (L-DOX) was 84%, and the liposomal diameter 96 nm. Less than 37% DOX was released after 48 h, and L-DOX could stably be stored for two weeks. L-DOX increased DOX toxicity by 1.8-4.6 times for osteosarcoma cell lines, and only 1.3 times for primary bone cells compared to free DOX, thus confirming higher sensitivity of OS cell lines vs. primary bone cells for L-DOX. Moreover, it was deduced that liposomal DOX passed more freely through the cell membrane than free DOX. Conclusion: We successfully synthesized a stealth L-DOX containing natural phospholipid by pH gradient method, and were able to encapsulate DOX with an 84% efficiency. The resulting nanoparticles were round-shaped, had suitable particle size, were stable for 2 weeks, showed adequate controlled DOX release, showed increased cell permeability compared to free DOX, and increased tumor cell kill, thus providing a novel, more effective therapeutic for OS treatment.
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نویسندگان مقاله | Fateme Haghiralsadat


| Ghasem Amoabediny


| Mohammad Hasan Sheikhha


| Tymour Forouzanfar


| Javad Mohammadnejad Arough


| Marco N Helder


| Behrouz Zandieh-doulabi


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4502
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