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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۳۷-۱۳۷
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عنوان انگلیسی Ps-106: Definitive Endoderm Differentiation of Wharton's Jelly Mesenchymal Stem Cells Using Signaling Molecules in Three Dimensional Scaffold
چکیده انگلیسی مقاله Objective: In this study, we focused on differentiating Wharton's Jelly mesenchymal stem cells into definitive endoderm lineage as the first step in differentiation into endoderm derivates, such as insulin-producing cells. Also, alginate capsules were utilized as a three dimensional scaffold. This system promotes cell to cell interactions that are essential for differentiation. Materials and Methods: An alginate solution was generated by dissolving of sodium alginate powder in NaCl solution. To create a cell-alginate mixture, Wharton's Jelly Mesenchymal Stem Cells were added to this solution and capsules were generated by extruding the mixture in a CaCl2 bath. Following encapsulation step, CaCl2 was removed and replaced with cell culture medium. Cells were decapsulated during culture for viability analysis. In order to induce solubilisation, capsules were incubated with a solution containing sodium citrate, then Trypan blue exclusion method was applied to determine cells viability. Encapsulated cells have been induced to definitive endoderm formation by Activin A and Wnt3a. To investigate the expression of definitive endoderm related genes, the RNA was extracted and Real-time PCR was performed. Results: The encapsulation procedure did not alter the morphology and viability of the enveloped cells. Post-differentiation analysis confirmed the expression of Sox17 and Foxa2, as definitive endoderm specific markers. Conclusion: Our results showed that alginate has potential to be used as a three dimensional scaffold for culturing and differentiation of Wharton's Jelly mesenchymal stem cells to definitive endoderm. Also using signaling molecules such as Activin A and Wnt3a could enhance the definitive endoderm differentiation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/431
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