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Veterinary Research Forum، جلد ۹، شماره ۴، صفحات ۲۹۳-۲۹۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی DNA damage in dental pulp mesenchymal stem cells: An in vitro study
چکیده انگلیسی مقاله The aim of this study was to evaluate the potential use of a DNA comet assay, DNA fragmentation fluorimetric assay and reactive oxygen species levels as potential biomarkers of genome conditions of dental pulp stem cells (DPSCs) isolated from dog canine teeth. Mesenchymal stem cells were isolated from the dental pulp collected from dog teeth. The results obtained suggest the ideal moment for clinical application of cellular therapy for this type of cell. The cell culture was maintained with Dulbecco's modified Eagle's medium supplemented with 10.00% fetal bovine serum for eight passages. During each passage, cell proliferation, oxidative stress and level of DNA fragmentation were assessed by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, testing 2,7 dichlorodihydro-fluorescein-diacetate and PicoGreen®, respectively. There were important differences among the first three DPSC passages compared to passages 4–8 and a large number of nuclei with some levels of DNA damage (30.00 to 40.00% in initial DPSC passages and > 50.00% in late passages), indicating in vitro DPSC genomic fragility. Within the limitations of this study, the results suggest these relatively simple and inexpensive approaches - comet and DNA fragmentation assays - could help sort stem cells with less DNA damage for use in research or therapies.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله Jaime Sardá Aramburú Junior |
Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil

Tiago Luis Eilers Treichel |
Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil

Saulo Tadeu Lemos Pinto Filho |
Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil

Sergio Alexandre Gerhke |
Biotecnos Research Center, Santa Maria, Rio Grande do Sul, Brazil; Catholic University of Uruguay, Montevideo, Uruguay

Alencar Kolinski Machado |
Morphology Department, Federal University of Santa Maria, Santa Maria, Brazil

Francine Carla Cadoná |
Morphology Department, Federal University of Santa Maria, Santa Maria, Brazil

Ivana Beatrice Mânica da Cruz |
Morphology Department, Federal University of Santa Maria, Santa Maria, Brazil

Ney Luis Pippi |
Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil

نشانی اینترنتی http://vrf.iranjournals.ir/article_33083_c01bfc358eca9ab478a456a2c88d0ff7.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/544/article-544-1112773.pdf
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