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Nanomedicine Journal، جلد ۱، شماره ۴، صفحات ۲۲۹-۲۳۷
عنوان فارسی
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عنوان انگلیسی Production of gold nanoparticles by Streptomyces djakartensis isolate B-5
چکیده انگلیسی مقاله Objective(s): Biosynthesis of gold nanoparticles (NGPs) is environmentally safer than chemical and physical procedures. This method requires no use of toxic solvents and synthesis of dangerous products and is environmentally safe. In this study, we report the biosynthesis of NGPs using Streptomyces djakartensis isolate B-5. Materials and Methods: NGPs were biosynthesized by reducing aqueous gold chloride solution via a Streptomyces isolate without the need for any additive for protecting nanoparticles from aggregation. We characterized the responsible Streptomycete; its genome DNA was isolated, purified and 16S rRNA was amplified by PCR. The amplified isolate was sequenced; using the BLAST search tool from NCBI, the microorganism was identified to species level. Results: Treating chloroauric acid solutions with this bacterium resulted in reduction of gold ions and formation of stable NGPs. TEM and SEM electro micrographs of NGPs indicated size range from 2- 25 nm with average of 9.09 nm produced intracellular by the bacterium. SEM electro micrographs revealed morphology of spores and mycelia. The amplified PCR fragment of 16S rRNA gene was cloned and sequenced from both sides; it consisted of 741 nucleotides. According to NCBI GenBank, the bacterium had 97.1% homology with Streptomyces djakartensis strain RT-49. The GenBank accession number for partial 16S rRNA gene was recorded as JX162550. Conclusion: Optimized application of such findings may create applications of Streptomycetes for use as bio-factories in eco-friendly production of NGPs to serve in demanding industries and related biomedical areas. Research in this area should also focus on the unlocking the full mechanism of NGPs biosynthesis by Streptomycetes.
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نویسندگان مقاله سارا بیگلری | sara biglari
department of agricultural biotechnology, college of agriculture, shahid bahonar university of kerman, iran

سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)

غلامحسین شهیدی | gholam hosein shahidi
department of plant pathology, college of agriculture, shahid bahonar university of kerman, iran

سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)

غلامرضا شریفی | gholam reza sharifi
department of agricultural biotechnology, college of agriculture, hormozgan university, iran

سازمان اصلی تایید شده: دانشگاه هرمزگان (Hormozgan university)

نشانی اینترنتی http://nmj.mums.ac.ir/article_2713.html
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زبان مقاله منتشر شده en
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