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Journal of Medical Bacteriology، جلد ۴، شماره ۵, ۶، صفحات ۱-۶
عنوان فارسی
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عنوان انگلیسی Determination of Extended-Spectrum Beta-lactamases Genes and Antibiotic Resistance Patterns in Escherichia coli Isolates from Healthy Cats
چکیده انگلیسی مقاله ne"> Background : This study was set to detect extended-spectrum beta-lactamases (ESBLs) producing E. coli isolates and the genes underlying their resistance in relation to phylogenetic background from fecal samples of healthy owned cats. Methods : A total of 50 E. coli isolates were confirmed by standard bacteriological tests. The phylogenetic analyses of the isolates were carried out by combinations of three genetic markers chuA , yjaA and DNA fragment TspE4.C2 by a triplex PCR method. The ESBL ( bla CTXM , bla TEM , bla SHV , bla OXA ) encoding genes were detected. To identify ESBL producing phenotypes, all selected isolates were screened with a double disk synergy test including cefotaxime, cefotaxime with clavulanic acid, ceftazidime and ceftazidime with clavulanic acid. Results : Results showed that E. coli isolates fell into four phylogenetic groups (A, D, B1 and B2) with prevalence of 78%, 4%, 8%, 10% and five phylogenetic subgroups including A0 (74 %), A1 (4 %), B1 (8 %), B2–2 (6 %), B2–3 (4 %) and D1 (4 %), respectively. Among all E. coli isolates, 4% were positive for bla SHV , bla CTX-M-15 and bla OXA-1 genes which distributed in B2-2, B2-3, A0 subgroups, respectively. According to antibiotic susceptibility test, 20 isolates were resistant which belonged to D (D1 phylogenetic subgroup) and A (A0 phylogenetic subgroup) groups. Conclusion : The results showed that healthy cats could be considered as potential source for the dissemination of ESBL-encoding genes. Further investigations in companion animals and their owners are needed to clarify the importance of spreading of these zoonotic strains.
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نویسندگان مقاله بهارک اختر | baharak akhtardanesh
department of clinical sciences, school of veterinary medicine, shahid bahonar university of kerman, kerman, iran.

سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)

قنبرپور | reza ghanbarpour
department of pathobiology, school of veterinary medicine, shahid bahonar university of kerman, kerman, iran.

سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)

المیرا یزدانی | elmira yazdani
department of clinical sciences, school of veterinary medicine, shahid bahonar university of kerman, kerman, iran.

سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)

نشانی اینترنتی http://jmb.tums.ac.ir/index.php/jmb/article/view/103
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نوع مقاله منتشر شده Original Articles
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