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Journal of Medical Bacteriology، جلد ۳، شماره ۳-۴، صفحات ۱-۷
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عنوان انگلیسی Comparison Study on Colonization of hilA Mutant and Parent Strains of Salmonella enteritidis in Vertically Infected Broiler Chickens
چکیده انگلیسی مقاله Background : Salmonella actively stimulates its own uptake into the epithelial cells by inducing cytoskeleton rearrangements and membrane ruffling triggered by some proteins secreted by Salmonella into the cytosol of the epithelial cells via a type III secretion system (TTSS) encoded by genes of the Salmonella pathogenicity island 1 (SPI-1). hilA is a transcriptional activator encoded on Salmonella Pathogenicity Island 1 (SPI-1) genes. Methods : To assess the importance of hilA in a simulation modeling of vertical infection and shedding of S. enteritidis in broiler chickens a long-term experiment was designed. Two groups of 200 fertile eggs were inoculated with 20 colony forming units (CFU) of hilA mutant of S. enteritidis or its parent strain just prior to incubation. Thirty five birds of each group were housed in separate rooms. On days 2, 4, 7, 14, 21, 28 and 35 of age, cloacal swabs from live birds as well as samples from internal organs (intestinal tract, liver and spleen) were evaluated by bacteriological or molecular methods. Results : In most of sampling days colonization and invasion of parent strain S. enteritidis in intestine (especially ceaca) and internal organs of chickens were higher with compared to its hilA mutant but this mutant strain could still colonize in intestinal tract and even invade liver or spleen. Conclusion : Colonization of hilA mutant of S. enteritidis indicated that hilA gene is only one part of the modulators in Salmonella invasion mechanism. The ability of hilA mutant to multiply and persist in host internal organs including ceaca may promise further research for potential of hilA mutant to prevent the initial colonization of the intestinal tract by a virulent S. enteritidis strain
کلیدواژه‌های انگلیسی مقاله Salmonella enteritidis, hilA mutant, Vertical, Broilers, PCR
نویسندگان مقاله محمدصادق مددی | mohammadsadegh madadi
department of clinical sciences, faculty of veterinary medicine, university of tabriz, tabriz, ir iran.

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

سارا میرزایی | sara mirzaie
department of animal, poultry and aquatic sciences, institute for agricultural research, iranian organization for science and technology, tehran, ir iran.


محمد حسن زاده | mohammad hassanzadeh
department of poultry diseases, faculty of veterinary medicine, university of tehran , ir iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://jmb.tums.ac.ir/index.php/jmb/article/view/24
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Articles
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