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International Journal of Fertility and Sterility، جلد ۱۳، شماره ۱، صفحات ۵۷-۶۵
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عنوان انگلیسی The Effects of Olive Leaf Extract on The Testis, Sperm Quality and Testicular Germ Cell Apoptosis in Male Rats Exposed to Busulfan
چکیده انگلیسی مقاله Objective: Busulfan (BU) has a destructive effect on the male reproductive system. On the one hand, traditional herbs such as olive leaf extract (OLE) have been considered as sources of antioxidants and phenolic compounds. The goal of this study was to assess the effects of OLE on BU-induced damages in rat testes. Materials and methods: In this experimental study, 40 male Wistar rats divided randomly into 5 groups. The control group (CTL) received dimethyl sulfoxide (DMSO), intraperitoneally (i.p.) for one time, which followed by administration of distilled water orally for 5 weeks. In BU group, BU (10 mg/kg) administrated i.p for one time. In co-treatment groups, at first mice received BU (10 mg/kg, i.p., and one time) and then OLE administrated orally at different doses of 250 mg/kg (BU+OLE 250), 500 mg/kg (BU+OLE 500) and 750 mg/kg (BU+OLE 750) for 5 weeks. Next, rats were sacrificed and blood and sperm were collected. Left testis was removed to investigate testicular parameters and apoptosis by using H&E and TUNEL staining, respectively. All data were analyzed by SPSS software, P < 0.05 consider as significant level. Results: There was a significant decline in sperm viability (p=0.017), number of primary spermatocyte (P=0.001) and Leydig cells (P=0.023) in the BU group versus the CTL group. Administration of OLE at three doses could repair these defects versus BU group Increasing in apoptotic spermatogonia cells (SG) due to BU was significantly reduced by administration of OLE at two doses of 250 and 500 mg/kg (P< 0.01). A reduction in germinal epithelium height and an increase in apoptotic SG were observed in BU+OLE 750 group versus all groups (P< 0.01). In BU+OLE 750 group, AST increased markedly in versus CTL (P=0.024) and also ALP was at a highest level. Conclusion: Oral administration of OLE in two doses 250 and 500 mg/kg could be helpful in modifying BU-induced toxicity in rat testes, while OLE at a dose of 750 mg/kg did not have these positive effects, even it could strengthen the harmful effects.
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نویسندگان مقاله | Sepideh Ganjalikhan Hakemi


| Fariba Sharififar ‎


| Tahereh Haghpanah


| Abdolreza Babaee


| Seyed Hassan Eftekhar-Vaghefi


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/5520
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