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Cell Journal، جلد ۲۰، شماره ۰۴، صفحات ۴۵۹-۴۶۸
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عنوان انگلیسی Amniotic Membrane Extract Eye Drop Promotes Limbal Stem Cell Proliferation and Corneal Epithelium Healing
چکیده انگلیسی مقاله Objective: Limbal stem cells (LSCs) are responsible for corneal epithelium reconstruction. Primary or acquired failure of LSCs leads to deleterious effects on corneal wound healing and its surface integrity. Much effort has been directed into finding substances that can accelerate LSC growth in vitro or in vivo. In the present study, we investigated the effects of human amniotic membrane extract as eye drops on human LSC proliferation and on corneal healing in a rabbit model. Materials and methods: This study was an interventional experimental study that used human amniotic membranes extract from healthy donors to enhance proliferation of LSCs in vitro and in vivo. In first step, to assess the effective and cytotoxic doses of AMMED, different concentrations of it (0-2.0 mg/ml) was added to human LSC cultures for 14 days. Subsequently, the expression levels of ATP-binding cassette sub-family G member 2 (ABCG2; a putative stem cell marker), cytokeratin 3 (K3) (corneal epithelial cell maker), K12 and K19 (corneal-conjuctival epithelial cell makers) were assessed using Real-Time PCR. In second step, the corneal epithelium of 10 rabbits was mechanically removed, and the right eye of each rabbit was treated with 1 mg/ml AMEED (every 2 hours and every 6 hours in different groups, respectively.) While there left eyes only were treated with an antibiotic (the control group). The corneal healing process, conjunctival infection, degree of eyelid edema, degree of photophobia and discharge scores was assessed daily. Finally, corneal tissues were biopsied for finding of pathologic evidences. Results: In comparison to positive control (10% FBS), AMEED at concentrations of 0.1–1 mg/ml induced LSC proliferation, upregulated ABCG2 (P< 0.05), and downregulated K3 (P< 0.05); but, there were no remarkable differences in the expression levels of K12 and K19 (P>0.05). Interestingly, in the rabbits treated with AMEED, the epithelium healing duration decreased from 4 days in the control group to 3 days in the two AMEED groups, with lower mean degrees of eyelid edema, chemosis, and infection compared to those in the control group. No pathologic abnormalities were observed in either of the AMEED groups. Conclusion: AMEED increases LSCs proliferation ex vivo and accelerates corneal epithelium healing in vivo, without any adverse effects. It could be used as supplement in LSCs expansion in cell therapy.
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نویسندگان مقاله | Niloufar Shayan Asl


| Farhad Nejat


| Parvaneh Mohammadi


| Abdolhossein Nekoukar


| Saeed Hesam


| Marzieh Ebrahimi


| Khosrow Jadidi


نشانی اینترنتی https://celljournal.org/journal/article/abstract/5423
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