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Cell Journal، جلد ۲۰، شماره ۰۴، صفحات ۵۱۳-۵۲۰
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عنوان انگلیسی Successful Human Spermatogonial Stem Cells Homing in Recipient Mouse Testis after In Vitro Transplantation and Organ Culture
چکیده انگلیسی مقاله Objective: SSCs (Spermatogonial stem cells) are considered the basis of spermatogenesis. This study investigated the homing of these cells in host tissues after transplantation to an azoospermia mouse testis model. Materials and methods: In this experimental study, human SSCs were isolated, and their identities were confirmed by tracking their PLZF (Promyelocytic Leukemia Zinc Finger) protein. These cells were transplanted to adult azoospermia mouse testes using two methods, namely, in vitro transplantation (IVT) and in vivo transplantation as transplantation groups, and testes without transplantation of cells were assigned in the control group. Then histomorphometric, immunohistochemical and molecular studies were done after 2 weeks. Results: After 2 weeks, histomorphometric studies revealed that the number of subsided SCs (Spermatogonial Cells) and the percentage of tubules with subsided SCs in IVT and in vivo groups were significantly more than those in the control group (P< 0.05). Immunohistochemical studies in the transplantation groups confirmed that the PLZF protein was expressed in the cells subsided on the seminiferous tubule. Real-time PCR demonstrated that the PLZF gene expression only in the transplantation groups was positive but was not significantly different between the IVT group and the in vivo group (P>0.05). Conclusion: Testicular tissue culture conditions after SSC transplantation can help these cells subside on the seminiferous tubule basement membrane.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Mahdi Mohaqiq


| Mansoureh Movahedin


| Zohreh Mazaheri


| Naser AmirJanati


نشانی اینترنتی https://celljournal.org/journal/article/abstract/5675
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