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Cell Journal، جلد ۲۰، شماره ۰۴، صفحات ۵۵۲-۵۵۸
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عنوان انگلیسی Anacardic Acid Reduces Acetylation of H4K12 in Mouse Oocytes during Vitrification
چکیده انگلیسی مقاله Objective: Vitrification has been widely used in recent times for oocyte cryopreservation, in animals and humans,which frequently causes minor and major epigenetic modifications. The effect of oocyte vitrification on acetylation of histone H4 at lysine 12 (AcH4K12) levels, and histone acetyltransferase (Hat) expression, has been assessed; however, little is known about the inhibition of Hat expression during the vitrification of oocytes. This study evaluated the effect of anacardic acid (AA) as a Hat inhibitor on vitrified mouse oocytes. Materials and methods: In this experimental study 248 MII mouse oocytes were used and divided in three experimental groups. 173 oocytes were selected and vitrified . 84 oocytes were vitrified without AA (vitrified group) and 89 oocytes were pretreated with AA, and then vitrified (treatment group). Mouse fresh MII oocytes used as control group. Hat expression and AcH4K12 levels were assessed by using real-time quantitative PCR and immunofluoresce staining, respectively. In addition, survival rate was analyzed in vitrified and treatment oocytes. Results: Hat activity and AcH4K12 modification increased significantly (respectively, 4.17±1.27, p≤0.001 and 97.57±6.30, p< 0.001) in oocytes that were vitrified, compared to the fresh oocytes. After treatment by AA, the Hat mRNA expression and subsequently H4K12 acetylation levels (respectively, 0.12±0.03, p≤0.001 and 89.79±3.20, p≤0.05) in the treated group were significantly reduced in comparison with the vitrified group (without AA). Furthermore, the survival rate was not significantly different between the vitrified (90.47%) and treatment (91.01%) groups (P>0.05). Conclusion: The present study suggests that AA reduces vitrification risks caused by epigenetics, but does not alter the quality of vitrification. In fact, the acetylation levels of H4K12 were reduced after treatment by AA.
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نویسندگان مقاله | Alaleh Ghazifard


| Mohammad Salehi


| Marefat Ghaffari Novin


| Mojgan Bandehpour


| Somayeh Keshavarzi


| Vahid Fallah Omrani


| Maryam Dehghani-Mohammadabadi


| Reza Masteri Farahani


| Ahmad Hosseini


نشانی اینترنتی https://celljournal.org/journal/article/abstract/5601
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