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Research in Molecular Medicine، جلد ۲، شماره ۱، صفحات ۳۵-۳۸
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عنوان انگلیسی Molecular Detection of Toxoplasma gondii Oocytes in the Soil from the Public Parks of the Arak City, Iran
چکیده انگلیسی مقاله Background: Soil structure is mainly composed of sand, silt, clay, and organic materials. Organisms can live in the soil. The large number of stray cats in the cities of Iran is a major environmental and health problem. Toxoplasma oocysts are shed with the feces of cats, so soil is known as a potential source of transmission of toxoplasmosis. The aim of this study was to determine the soil contamination with Toxoplasma gondii oocyst in the public parks of the Arak city. Materials and Methods: Sixty soil samples were collected from 15 main parks of Arak city. Four soil samples from the children's playground, a potting place, around trash bins, and around toilets were taken. Oocyst was isolated from soil by floatation in saturated sucrose. Floating debris was tested by two methods: staining by the modified Ziehl-Neelsen technique and PCR. The target of PCR was the 122 bp fragment of the B1 gene.. Results: From 60 soil samples of public parks of Arak city, 8 samples (13%) were suspected to Toxoplasma oocyst contamination in staining smears. Only 3 samples (5%) of 60 samples were positive in PCR. The results showed that the staining method is not a good method to detect oocysts in the soil because the diversity of oocyst in soil is very high and similar in appearance. Conclusion: This study showed soils of public parks in the Arak city were contaminated to oocyst of Toxoplasma. Also molecular method for the detection of parasites in the soil was more suitable than staining method.
کلیدواژه‌های انگلیسی مقاله Oocyst, PCR, Soil, Toxoplasma gondii
نویسندگان مقاله حدیث solymane | hadis solymane
ponak, hesarak,lamic azad university, science and research branch, tehran, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

زهرا اسلامی راد | zahra eslamirad
sardasht, basij square, mojtame pardis, school of medical sciences, arak, iran


منصور بیات | mansour bayat
ponak,hesarak,lamic azad university, science and research branch, tehran, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

رضا حاجی حسین | reza hajihossein
sardasht, basij square, mojtame pardis, school of medical sciences, arak, iran
نشانی اینترنتی http://rmm.mazums.ac.ir/browse.php?a_code=A-10-642-1&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده انگل شناسی
نوع مقاله منتشر شده پژوهشی
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