این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Research Journal of Pharmacognosy، جلد ۳، شماره ۳، صفحات ۱-۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Development and validation of a HPLC-UV method for determination of Proscillaridin A in Drimia maritima
چکیده انگلیسی مقاله Background and objectives:Drimia maritima (L.) Stearn also known as squill is a medicinally important plant that has been used for various ailments such as dropsy, respiratory disorders, jaundice and epilepsy from ancient times. Proscillaridin A is identified as one of the most effective compounds in the plant with remarkable pharmacological features including efficacy against congestive heart failure, antitumor, t-cell suppressive and analgesic activities. In the present study, a reliable high performance liquid chromatography (HPLC) method has been developed for quantification of proscillaridin A in D. maritima. Methods: The separation of compounds was performed using gradient elution (methanol: water) on a reversed phase ACE C18 with flow rate of 1 mL/min and UV detection at 300 nm for 50 min. The method was evaluated using validation parameter such as selectivity, linearity, precision, recovery, limit of detection (LOD) and limit of quantization (LOQ). Results: The separation technique was selective for quantification of proscillaridin A. The calibration graph was linear with r2> 0.998. The intra and inter-day precision (RSD%, 3.8-4.16 and 7.5) were satisfactory. LOD and LOQ were calculated as 0.6 and 1.8 µg/mL respectively. The recovery average was 93.7%. Conclusion: Due to precision, accuracy and speed, the proposed HPLC-UV method could be applied for determination of proscillaridin A in Drimia maritima samples.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله m بزرگی |
department of traditional pharmacy, faculty of traditional medicine, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

gh امین |
department of pharmacognosy, faculty of pharmacy, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

s kasebzade |
pharmaceutical sciences branch, islamic azad university, tehran, iran.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

m شکارچی |
food and drug laboratory research center, food and drug control laboratories, moh and me, tehran, iran.

نشانی اینترنتی http://www.rjpharmacognosy.ir/article_15629_999bb4f14b54d4994d0b8b9237af60da.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات