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Journal of Medical Microbiology and Infectious Diseases، جلد ۳، شماره ۱، صفحات ۰-۰
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عنوان انگلیسی An In Vitro Study on Impact of Environmental Stresses on Growth, Morphological and Biochemical Features of Listeria monocytogenes PTCC 1297
چکیده انگلیسی مقاله Introduction: Listeria monocytogenes is a serious concern for the food industry due to its high case fatality rate, widespread distribution, ability to survive a wide variety of food processing conditions, and the severity of the illness associated with this pathogen infection. The objective of this study was to determine the growth, cell morphology and biochemical characteristics of L. monocytogenes PTCC 1297 (Serotype 4a) under selected environmental stresses. Method: The environmental stresses were acid stress (HCl, pH 2.0-6.0), alkaline stress (NaOH, pH 8.0-12.0), ethanol stress (5.0%-25.0% vol/vol), oxidative stress (H2O2, 0.06%-6.0% vol/vol), osmotic stress (NaCl and sucrose, 2.0%-30.0% wt/vol) and heat stress (40-60°C). All stresses were applied to the exponential phase bacteria whereas non-stressed exponential phase cells served as a control and the cells were allowed to grow for 24 h. For evaluating the growth of L. monocytogenes PTCC 1297 after inoculation procedure and exposure of cells to selected stresses we used colony count method. Scanning electron microscopy (SEM) was implemented to visualize the external appearance of the bacteria. Results: According to the results, the bacteria at pH≤4 and pH≥10 achieved by HCl and, NaOH, respectively, died. Also, concentrations ethanol at ≥15% vol/vol, H2O2 ≥0.3% vol/vol, NaCl ≥14% wt/vol, and heat ≥50°C were lethal for the bacteria. Unlike other stresses, sucrose did not kill bacteria but decreased their growth. The phenotypical and biochemical characteristics of them changed when exposed to each stress. Conclusion: Different doses of various stresses were either lethal or sub-lethal for this bacterium and lead to various changes in its characteristics.
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نویسندگان مقاله سمانه کاظمی | samaneh kazemi
vice-chancellor of research and technology, guilan university of medical sciences, rasht, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گیلان (Guilan university of medical sciences)

محمد فایزی قاسمی | mohammad faezi ghasemi
department of microbiology, islamic azad university, lahijan branch, lahijan, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-92-1&slc_lang=en&sid=en
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Other
نوع مقاله منتشر شده Original article
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