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Journal of Medical Microbiology and Infectious Diseases، جلد ۳، شماره ۱، صفحات ۰-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Antibacterial Effects of Aloe Vera Extracts on some Human and Animal Bacterial Pathogens
چکیده انگلیسی مقاله Introduction: Aloe Vera compounds have inhibitory activity on fungi, bacteria, and viruses. This study examines the antibacterial activity of A. Vera purified extracts including gel, boiled skin, boiled gel, and distilled extract against pathogenic bacteria, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Klebsiella pneumonia and Pseudomonas aeruginosa were elucidated. Method: The bacterial strains were collected from veterinary hospital. Freshly collected A. vera leaves were used for the juice extraction of gel, skin and distilled extracts. Antibacterial effects of various A. Vera extracts were evaluated using broth microdilution method. The crude polysaccharides of boiled skin extract were purified by phenol method; and fractionated by anion exchange chromatography. For each bacterium, minimum inhibitory concentration of various A. Vera extracts was determined. The protein expression changes of treated bacteria were detected by SDS-PAGE electrophoresis. Results: The distillate extract exhibited more antibacterial effects than other extracts. Out of seven-carbohydrate fractions of the skin extract, the fractions 6 and 7 had antibacterial effects on S. aureus and MRSA at 0.089 and 0.134 mg/ml, respectively; also fraction 5 showed antibacterial effects on MRSA at 0.113 mg/ml concentration. The protein profiles of these strains before and after treatment with A. Vera showed significant differences at 175, 60, 200 and 70 kDa protein bands of S. aureus, MRSA, P. aeruginosa and K. pneumonia, respectively. Conclusion: This finding showed that the distillate extract despite the minimal amount of carbohydrate and protein was more efficient against both Gram-positive and Gram negative bacteria.
کلیدواژه‌های انگلیسی مقاله Aloe Vera, Extracts, Antibacterial
نویسندگان مقاله داریوش غریبی | darioush gharibi
faculty of veterinary medicine, shahid chamran university of ahvaz, khuzestan, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

محمد خسروی | mohammad khosravi
faculty of veterinary medicine, shahid chamran university of ahvaz, khuzestan, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

زهره حسینی | zohreh hosseini
faculty of veterinary medicine, shahid chamran university of ahvaz, khuzestan, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

فاطمه برون | fatemeh boroun
faculty of veterinary medicine, shahid chamran university of ahvaz, khuzestan, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

سیده کلثوم برزگر | seyedeh kolsum barzgar
faculty of veterinary medicine, shahid chamran university of ahvaz, khuzestan, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

علی فروغی فرد | ali forughi far
faculty of veterinary medicine, shahid chamran university of ahvaz, khuzestan, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-89-2&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Anti-microbial agents, resistance and treatment protocols
نوع مقاله منتشر شده Original article
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