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Journal of Medical Microbiology and Infectious Diseases، جلد ۲، شماره ۳، صفحات ۱۲۱-۱۲۴
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عنوان انگلیسی Employing PCR Technique in Assessment of Monoclonality in Large B-cell Non-Hodgkin's Lymphoma
چکیده انگلیسی مقاله Introduction: Most B-cell malignancies are diagnosed based on morphologic and immunohistochemical criteria. Some cases, however, still present a challenge for the pathologist to discriminate between reactive hyperplasia and neoplastic disorders. Molecular techniques can be used as a helpful diagnostic tool in these cases. In this study, we assessed the value of polymerase chain reaction (PCR) technique in determination of monoclonality of immunoglobulin heavy chain gene rearrangements for the diagnosis of large B-cell non-Hodgkin's lymphoma (NHL) in paraffin embedded tissue samples. Methods: DNA was extracted from paraffin embedded tissues of 44 diffuse large B-cell lymphoma (DLBCL) cases and 20 samples of reactive lymphoid tissues from appendix and tonsils as control. Framework 3 and the joining region (FR3/JH) of the variable segment of the immunoglobulin heavy chain gene were amplified using degenerated primers. PCR products from each sample were analyzed on 8% polyacrylamide gels following AgNO3 staining. Results: Monoclonal rearrangements were identified in 33 of 44 cases (75%) of DLBCL using FR3/JH primers. Monoclonal IgH gene rearrangements were not detected in any of the reactive lymphoid hyperplasic samples. All these control cases showed polyclonal pattern. Conclusion: Through PCR analysis, using degenerated primers, monoclonality was demonstrated in 75% of DLBCL cases. This technique can thus be used as a sensitive, reliable and valuable diagnostic supplement to conventional morphologic examination and immunohistocytochemical evaluation of lymphoproliferative disorders, particularly in cases with restrictions in amount or type of analytic material.
کلیدواژه‌های انگلیسی مقاله Immunoglobulin Gene, PCR, Non-Hodgkins Lymphoma
نویسندگان مقاله نوشین لطفی | noushin lotfi
mashhad, iran


مریم راستین | maryam rastin
mashhad, iran


پریسا شعاعی | parisa shoaei
isfahan,iran


بهرام معمار | bahram memar
mashhad, iran


نفیسه سادات طبسی | nafiseh sadat tabasi
mashhad, iran


زهره محمودی | zohreh mahmoudi
mashhad, iran


رضا علی محمدی | reza alimohammadi
mashhad, iran


بهنام یوسفی | behnam yousefi
mashhad, iran


محمود محمودی | mahmoud mahmoudi
mashhad, iran
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-48-2&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Immune responses, deficiencies and vaccine candidates
نوع مقاله منتشر شده Original article
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