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Iranian Biomedical Journal، جلد ۲۳، شماره ۲، صفحات ۱۵۹-۱۶۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Comparison of OmpA Gene-Targeted Real-Time PCR with the Conventional Culture Method for Detection of Acinetobacter baumanii in Pneumonic BALB/c Mice
چکیده انگلیسی مقاله Background: Acinetobacter baumannii is an important pathogen in health care and is responsible for severe nosocomial and community-acquired pneumonia. To design novel therapeutic agents, a mouse model for A. baumannii pneumonia is essential. Methods: We described a mouse model of A. baumannii using clinical and 19606R standard strains for developing a quantitative real-time PCR (qRT-PCR) for rapid identification of A. baumannii infection from lung tissues of BALB/c mice. Results: To infect the mice, three doses of bacteria (0.5 × 108, 1 × 108, and 1.5 × 108 cfu/ml) were used. Lung tissues were cultured and compared with ompA gene. Clinical isolate had better positive results at day three with the highest dose than 19606 strain either in culture (4 versus 3) or in qRT-PCR (5 versus 4). However, qRT-PCR detection was 100%, the specificity was 70%, and the positive predictive value was 27%. Conclusion: The qRT-PCR detection of A. baumannii in the BALB/c mice model had a high sensitivity than culture method.
کلیدواژه‌های انگلیسی مقاله Acinetobacter baumanii, OmpA, qRT-PCR Pneumonia, BALB/c mice
نویسندگان مقاله | Niloofar Hassannejad
Dept. of Cell Biology, Science and Research branch, Islamic Azad University, Tehran, Iran


| Abbas Bahador
Dept. of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Nasim Hayati Rudbari
Dept. of Cell Biology, Science and Research branch, Islamic Azad University, Tehran, Iran


| Mohammad Hossein Modarressi
Dept. of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran


| Kazem Parivar
Dept. of Cell Biology, Science and Research branch, Islamic Azad University, Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-770&slc_lang=en&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/125/article-125-1206719.pdf
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زبان مقاله منتشر شده en
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