این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Biotechnology، جلد ۱۴، شماره ۳، صفحات ۱۰۹-۱۱۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Analysis of Promyelocytic Leukemia in Human Embryonic Carcinoma Stem Cells During Retinoic Acid-Induced Neural Differentiation
چکیده انگلیسی مقاله Background: Promyelocytic leukemia protein (PML) is a tumor suppressor protein that is involved in myeloid cell differentiation in response to retinoic acid (RA). In addition, RA acts as a natural morphogen in neural development. Objectives: This study aimed to examine PML gene expression in different stages of in vitro neural differentiation of NT2 cells, and to investigate the possible role of PML in pluripotency and/or neural development. Materials and Methods: RA was used as a neural inducer for in vitro neural differentiation of NT2 cells. During this process PML mRNA and protein levels were assessed by quantitative real time RT-PCR (QRT-PCR) and Immunoblotting, respectively. Furthermore bisulfite sequencing PCR (BSP) was used to assess PML promoter methylation in NT2 cells and NT2 derived neuronal precursor cells (NT2.NPCs). Results: QRT-PCR results showed that, PML had maximum expression with significant differences in NT2 derived neuronal precursor cells relative to NT2 cells and NT2 derived neural cells (NT2.NCs). Numerous isoforms of PML with different intensities appeared in immunoblots of pluripotent NT2 cells, NT2.NPCs, and NT2.NCs. Furthermore, the methylation of the PML promoter in NT2.NCs was 2.6 percent lower than NT2 cell. Conclusions: The observed differences in PML expression in different cellular stages possibly could be attributed to the fact that PML in each developmental state might be involved in different cell signaling machinery and different functions. The appearance of different PML isoforms with more intensity in neural progenitor cells; may suggest apossible role for this protein in neural development.
کلیدواژه‌های انگلیسی مقاله Pluripotent stem cells,Promyelocytic Leukemia,retinoic acid
نویسندگان مقاله خدیجه کربلایی |
division of genetics, department of biology, faculty of science, university of isfahan, isfahan, iran department of cellular biotechnology, cell science research center, royan institute for biotechnology, acecr, isfahan, iran
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

صادق ولیان |
division of genetics, department of biology, faculty of science, university of isfahan, isfahan, iran
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

لیانا لچینانی |
department of cell and molecular biology, cell science research center, royan institute for biotechnology, acecr, isfahan, iran
سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

سمیه تنهایی |
department of molecular genetics , cell science research center, royan institute for biotechnology, acecr, isfahan, iran
سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

حسین بهاروند |
department of stem cells and developmental biology, cell science research center, royan institute for stem cell biology and technology, acecr, tehran, iran department of developmental biology, university of science and culture, tehran, iran
سازمان اصلی تایید شده: دانشگاه علم و فرهنگ (University of science and culture)

محمد حسین نصر اصفهانی | mohammad hossein nasr esfahani
department of cellular biotechnology, cell science research center, royan institute for biotechnology, acecr, isfahan, iran
سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

نشانی اینترنتی http://www.ijbiotech.com/article_15459_a5fb1dd765919f1218eafee7cff3ee87.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات