این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Biotechnology، جلد ۱۳، شماره ۳، صفحات ۱۰-۱۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Bacterial Secretome Analysis in Hunt for Novel Bacteriocins with Ability to Control Xanthomonas citri subsp. Citri
چکیده انگلیسی مقاله Background: Xanthomonas citri subsp. citri (Xcc), the causative agent of bacterial citrus canker, has affected citriculture worldwide. Varieties of means have been used to minimize its devastating effects, but no attention has been given to bacteriocins. Objectives: Here and for the first time, we report the isolation and characterization of two novel bacteriocins. Materials and Methods: Secretome containing bacteriocins of isolated bacteria was separated via SDS-PAGE. Each isolated protein band was characterized and checked for its efficacy in controlling two pathogenic isolates of Xcc via disk diffusion assay. The effects of varieties of carbon, nitrogen and phosphate sources were evaluated on both bacterial growth and bacteriocin production via Taguchi orthogonal method. Results: The two bacteriocins showed an activity up to 55ºC that were sensitive to proteases suggesting being protein in nature. Analysis of SDS-PAGE purified protein bands of bacterial secretomes with demonstrated potency against Xcc revealed the presence of peptides with relative molecular masses of 16.9 and 17 kDa for Cronobacter and Enterobacter, respectively. Sequence analysis of peptides revealed an HCP1 family VI secretion system homologue for Cronobacter (YP_001439956) and pilin FimA homologue for Enterobacter (CBK85798.1). A Taguchi orthogonal array was also implemented to determine the effect of temperature and eight other chemical factors on bacteriocin production for each bacterium. Conclusions: Two peptides with novel antibacterial activities effective against Xcc were isolated, characterized and conditions were optimized for their higher production.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله داریوش غلامی |
national institute of genetic engineering and biotechnology
سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

طناز گودرزی |
national institute of genetic engineering and biotechnology
سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

سعید امین زاده |
national institute of genetic engineering and biotechnology
سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

سید مهدی علوی | seyed mehdi
national institute of genetic engineering and biotechnology
سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

نسرین کاظمی پور |
school of veterinary medicine, shiraz university, shiraz, iran
سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

ناصر فرخی |
biotechnology research department department of agronomy and plant breeding, shahrood university
سازمان اصلی تایید شده: دانشگاه شاهرود (Shahrood university)

نشانی اینترنتی http://www.ijbiotech.com/article_10993_36fa61f44055bff44e07787340c198a1.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات