این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Biotechnology، جلد ۱۳، شماره ۲، صفحات ۱۰-۱۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Cloning and expression of Iranian Turkmen-thoroughbred horse follicle stimulating hormone in Pichia pastoris
چکیده انگلیسی مقاله Background: Follicle stimulating hormone (FSH) plays an essential role in reproductive physiology and follicular development. Objective: A new variant of the equine fsh (efsh) gene was cloned, sequenced, and expressed in Pichia pastoris (P. pastoris) GS115 yeast expression system. Materials and Methods: The full-length cDNAs of the efshα and efshβ chains were amplified by reverse transcription polymerase chain reaction (RT-PCR) using the total RNA isolated from an Iranian Turkmen-thoroughbred horse's anterior pituitary gland. The amplified efsh chains were cloned into the pPIC9 vector and transferred into P. pastoris. The secretion of recombined eFSH using P. pastoris expression system was confirmed by Western blotting and immunoprecipitation (IP) methods. Results: The DNA sequence of the efshβ chain accession number JX861871, predicted two putative differential nucleotide arrays, both of which are located in the 3′ UTR. Western blotting showed a molecular mass of 13 and 18 kDa for eFSHα and eFSHβ subunits, respectively. The expression of desired protein was confirmed by protein G immunoprecipitation kit. Conclusions: eFSH successfully expressed in P. pastoris. These findings lay a foundation to improve ovulation and embryo recovery rates as well as the efficiency of total embryo-transfer process in mares.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله زهرا الیاسی گرجی | elyasi gorji
department of genetics, reproductive biomedicine research center, royan institute for reproductive biomedicine, acecr, tehran, iran department of animal breeding, genetics and physiology, gorgan university of agricultural sciences and natural resources, gorgan, iran
سازمان اصلی تایید شده: دانشگاه گلستان (Golestan university)

امیر امیری یکتا | amiri yekta
department of genetics, reproductive biomedicine research center, royan institute for reproductive biomedicine, acecr, tehran, iran
سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

حمید گورابی |
department of genetics, reproductive biomedicine research center, royan institute for reproductive biomedicine, acecr, tehran, iran
سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

سعید حسنی |
department of animal breeding, genetics and physiology, gorgan university of agricultural sciences and natural resources, gorgan, iran
سازمان اصلی تایید شده: دانشگاه گلستان (Golestan university)

nayeralsadat فاطمی |
department of genetics, reproductive biomedicine research center, royan institute for reproductive biomedicine, acecr, tehran, iran.
سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

سعید زره داران |
department of animal breeding, genetics and physiology, gorgan university of agricultural sciences and natural resources, gorgan, iran
سازمان اصلی تایید شده: دانشگاه گلستان (Golestan university)

فایزه vakhshiteh |
institute of bioscience, universiti putra malaysia, serdang, selangor, malaysia

محمد حسین صنعتی | mohammad hossein
department of genetics, reproductive biomedicine research center, royan institute for reproductive biomedicine, acecr, tehran, iran,
سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

نشانی اینترنتی http://www.ijbiotech.com/article_11310_2da2927b8c50a33c6488e86b6783befe.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات