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Iranian Journal of Biotechnology، جلد ۱۳، شماره ۱، صفحات ۳۶-۴۲
عنوان فارسی
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عنوان انگلیسی Identification of Crocus sativus and its Adulterants from Chinese Markets by using DNA Barcoding Technique
چکیده انگلیسی مقاله Background: Saffron (Crocus sativus L.) is a common but very expensive herbal medicine. As an important traditional medicine, it has an outstanding effect in treating irregular and painful menstruation. Recently, the over-demand tendency of saffron results in an unusual phenomenon in the medicinal markets. Adulterants and saffron-like substitutes are intentionally mixed into medicinal markets and pharmacies or online stores, affecting drug safety and food quality. Objectives: Our study aimed to identify saffron from its adulterants via DNA barcoding. Materials and Methods: Samples (13 saffron + 4 others containing Carthamus tinctorius or Chrysanthemum x morifolium) obtained from 12 different provinces of China. Through DNA barcoding, samples were compared using three candidate markers, trnH-psbA, rbcL-a and ITS2. Results: trnH-psbA and rbcL-a were capable of distinguishing different accessions. ITS2 could identify samples even at intra-specific level. According to these three barcodes, four samples were identified saffron-like substitutes. Conclusions: The adulterant rate in Chinese markets reaches as high as 33.33% that may cause health risks and further may reduce saffron efficacy once is being used as herbal remedy. In order to make a distinction between C. sativus with other genera as adulterants, DNA barcoding is suggested.
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نویسندگان مقاله weijuan هوانگ |
department of biology, college of life and environmental sciences, minzu university of china, beijing 100081, pr china

feifei li |
department of biology, college of life and environmental sciences, minzu university of china, beijing 100081, pr china

yujing liu |
department of biology, college of life and environmental sciences, minzu university of china, beijing 100081, pr china

chunlin لنگ |
1-department of biology, college of life and environmental sciences, minzu university of china, beijing 100081, pr china 2-kunming institute of botany, chinese academy of sciences, kunming 650201, pr china

نشانی اینترنتی http://www.ijbiotech.com/article_9811_5b7b37efebae69acf10cbca26bd8119e.pdf
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