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Iranian Journal of Biotechnology، جلد ۱۲، شماره ۲، صفحات ۳۲-۴۰
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عنوان انگلیسی Molecular Characterization and Antimicrobial Resistance of Uropathogenic Escherichia coli
چکیده انگلیسی مقاله Background:Urinary Tract Infections (UTIs) are the most common infectious diseases in childhood. The Uropathogenic Escherichia coli (UPEC) strains account for as much as 80% of UTIs. Objective:From a clinical perspective, it is important to know which virulence factors and antibiotic resistance properties are present in UPEC strains in pediatrics. Therefore, this study was carried out to investigate the molecular characterization and antimicrobial resistance of UPEC strains isolated from hospitalized patients in pediatric ward of Baqiyatallah Hospital in Tehran. Patients and Methods:One hundred and twenty-one urine specimens were collected from the patients infected with UTIs (51 boys and 70 girls). The urine samples were cultured immediately, and those with E. coli-positive were analyzed for the presence of antibiotic resistance genes and bacterial virulence factors using Polymerase Chain Reaction (PCR). Also, antimicrobial susceptibility testing was performed using disk diffusion methodology with Mueller–Hinton agar according to the instruction of Clinical Laboratory and Standard Institute. Results:Nineteen out of 51 (37.25%) urine samples from boys and 47 out of 70 (67.14%) urine samples from girls harbored E. coli. A significant difference was found between the frequency of UPEC strains in boys and girls (P
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نویسندگان مقاله فاطمه مشایخی |
school of nursing and midwifery, jiroft university of medical sciences, jiroft, i.r. iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی جیرفت (Jiroft university of medical sciences)

ماندانا مغنی |
department of clinical pathology, shahrekod university of medical science, shahrekord, i.r. iran

مطهره فرامرزپور |
school of nursing and midwifery, jiroft university of medical sciences, jiroft, i.r. iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی جیرفت (Jiroft university of medical sciences)

عماد یاحقی |
baqiyatallah university of medical sciences, tehran, i.r. iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

ابراهیم خداوردی داریان | khodaverdi darian
young researchers and elite club, karaj branch, islamic azad university, karaj, i.r. iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

وحیده tarhriz |
department of pharmaceutical biotechnology, faculty of pharmacy, tabriz university of medical sciences,tabriz, i.r. iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

بنفشه درمنش |
department of pediatric nephrology, aja university of medical sciences, tehran, i.r. iran

نشانی اینترنتی http://www.ijbiotech.com/article_7246_c39939315203d7b298c096899006dbb7.pdf
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