این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Biotechnology، جلد ۱۲، شماره ۱، صفحات ۳۰-۳۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Production and Purification of Equine Chorionic Gonadotropin Hormone Using Polyclonal Antibody
چکیده انگلیسی مقاله Background: Equine chorionic gonadotropin (eCG) is commonly used in association with a progestagen treatment to synchronize estrus of goats and ewes during breeding and non-breeding seasons. Classical purification of the eCG from serum includes pH fractionation with metaphosphoric acid two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. Objectives: The aim of the current study was to develop an accurate and fast method for production and purification of eCG using polyoclonal antibody assay. Materials and Methods: The blood samples (300 ml) were taken from jugular vein of 17 mares on days 50, 70 and 90 of pregnancy. Plasma of the samples was siphoned and phenol solution has been added to the plasma and stored in the refrigerator until eCG extraction. To prepare polyoclonal antibody against eCG, four male rabbits, about 4-months-old and 2-kg weights, were chosen. A basic immunization was done by injecting 25 IU of eCG to the rabbits. Ouchterlony assay or double immunodiffusion test was used to assess the immunization and the titer of antiserum against eCG. eCG has been purified from the plasma via Solid-Phase Extraction (SPE) method. Results: Results based on agarose-gel double immunodiffusion test showed that rabbits have been completely immunized. SDS-PAGE analysis showed purified eCG is extracted without any significant contamination. Conclusions: The extraction of eCG with polyclonal antibody using SPE method and production of anti- eCG antiserum in rabbits is suitable and may be a cost effective method for large scale production of eCG and anti- eCG antiserum in Iran.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله محمد علی شریف | mohammad ali
department of animal science, faculty college of agriculture and natural resources, university of tehran, karaj, i.r. iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

حمید کهرام |
department of animal science, faculty college of agriculture and natural resources, university of tehran, karaj, i.r. iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

احمد زارع شحنه | zare shahneh
department of animal science, faculty college of agriculture and natural resources, university of tehran, karaj, i.r. iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

حسین ذوالفقاریان |
razi vaccine amp;amp; serum research institute, karaj, i.r. iran

بهمن عابدی کیاسری | abedi kiasari
razi vaccine amp;amp; serum research institute, karaj, i.r. iran

مهدی هدایتی |
cellular amp;amp; molecular research center, research institute for endocrine research center, shahid beheshti university of medical sciences, tehran, i.r. iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

نشانی اینترنتی http://www.ijbiotech.com/article_7249_5994bc5ee9d68374bd7eec63043fa862.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات