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Iranian Journal of Biotechnology، جلد ۱۱، شماره ۳، صفحات ۱۶۸-۱۷۴
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عنوان انگلیسی Heterologous Expression of Human IL-29 (IFN-λ1) in Iranian Lizard Leishmania
چکیده انگلیسی مقاله Background: Interferons with diffrent functions such as antiviral, antiproliferative and immunomodulatory actions are effctive medications for a number of diseases. One of these new interferons (IFNs) is Interleukin-29 (IL-29) belongs to the family of IFN-λ has antiviral activity and its potent in accompanying with IFN-α in treatment of HCV infection has been evaluated (clinical trial phase II). Recombinant IL-29 has been previously produced in multiple expression systems but in this study we cloned and expressed this protein in an Iranian Lizard leishmania (I.L.L) for the fist time. Objectives: The Main objective of this research was to evaluate expression of functional human IL-29 in domestic Lizard leishmania as an alternative eukaryotic expression system. Materials and Methods: The IL-29 expression cassette was constructed into a pLEXSY vector. The leishmania cells were transfected by electroporation. After selection of transfectants, the protein expression was evaluated at RNA and protein levels. Results: Expression cassette was successfully transfected to leishmania cells and expression of recombinant IL-29 was proved by RT-PCR and western blotting. Purifid protein showed 20% activity compared to standard protein. Enzymatic removal of N-glycan resulted to the shift of protein mobility on SDS-PAGE. Conclusions: Easy handling and culture of this eukaryotic host and mammalian cell like posttranslational modifiations are the main advantages of this expression host, but the expression yield of this protein is very low and it seems to be not economic for large-scale production.
کلیدواژه‌های انگلیسی مقاله Interferons,Interleukin-29,Recombinant,Leishmania
نویسندگان مقاله امیرحسین طارم چی | amir hossein
biotechnology department, shahid beheshti university of medical sciences, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

بهرام کاظمی |
biotechnology department, shahid beheshti university of medical sciences, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

ساناز مهمازی |
department of genetics, zanjan branch, islamic azad university, zanjan, ir iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

مژگان بنده پور |
biotechnology department, shahid beheshti university of medical sciences, tehran, ir iran and cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

نشانی اینترنتی http://www.ijbiotech.com/article_7218_e4c948b3e20046908197e28ea1a08947.pdf
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