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Iranian Journal of Biotechnology، جلد ۱۱، شماره ۱، صفحات ۳۲-۴۰
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عنوان انگلیسی Characterization and Potentials of Indigenous Oil-Degrading Bacteria Inhabiting the Rhizosphere of Wild Oat (Avena Fatua L.) in South West of Iran.
چکیده انگلیسی مقاله Background: The contamination of ecosystem with petroleum and its derivatives is con­sidered as one of the most crucial environmental threat in Iran. Application of micro­organisms has been demonstrated as an appropriate and more practical alternative to clean-up petroleum hydrocarbons in the contaminated environments. Objectives: The objectives of this study were isolating rhizosphere-inhabiting indig­enous oil-degrading bacteria in wild oat grown in petroleum-polluted areas and in vitro evaluating the efficiency of oil biodegradability by microbial isolates. Materials and Methods: Bacteria were isolated from rhizosphere of wild oat grown on contaminated sites in Khuzestan and were identified based on 16S rRNA gene sequenc­ing. The catabolic genes were detected using PCR and hybridization analysis. Hydrocar­bon degradation in liquid culture was evaluated by gas chromatography-mass spec­trometry (GC-MS). Results: 23 indigenous oil-degrading bacterial strains were isolated from the wild oat rhizosphere, grown in severely oil contaminated soil in Khuzestan. Based on 16S rRNA gene sequence analyses, isolated strains were classified to Genera Acinetobacter, Pseudo­monas, Enterobacter, Stenotrophomonas, Bacillus, Achromobacter, Ochrobactrum, Paenibacil­lus, Microbacterium, Curtobacterium and Sphingobacterium. Catabolic genes alkM, alkB and xylE, responsible for biodegradation of the alkanes and aromatic petrochemical com­pounds were detected in bacterial community inhabiting rhizosphere of the wild oat. The GC-MS analysis indicated that consortium of these bacteria was capable of reducing crude petroleum in the liquid culture by 40.5%, after 10 days. The results of the present study revealed the adaptability of microbes to the rhizospheric area and subsequently their great potential to be exploited for cleaning up hydrocarbon contaminated sites. Conclusions: This study might be an important step towards the development of a phy­toremediation strategy in the South of Iran.
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نویسندگان مقاله سعیده رجایی |
soil science department, faculty of agriculture, shahrekord university, shahrekord, ir iran

سید مهدی سیدی | seyed mahdi
department of plant biotechnology, national institute of genetic engineering and biotechnology, tehran, ir iran
سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

فایز رییسی |
soil science department, faculty of agriculture, shahrekord university, shahrekord, ir iran

بهروز شیران |
department of agronomy and plant breeding, faculty of agriculture, shahrekord university, , shahrekord, ir iran

جمشید راهب |
department of industrial and environmental biotechnology, national institute of genetic engineering and biotechnology, tehran, ir iran
سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

نشانی اینترنتی http://www.ijbiotech.com/article_7197_befc9cd5a5187954de33d093ad06bf95.pdf
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