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Iranian Journal of Biotechnology، جلد ۸، شماره ۲، صفحات ۷۳-۸۱
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عنوان انگلیسی Transformation of Potato (Solanum tuberosumcv.Savalan) by Chitinase and β-1,3-Glucanase Genes of Myco-Parasitic Fungi Towards Improving Resistance to Rhizoctonia solani AG-3
چکیده انگلیسی مقاله Potato (Solanum tuberosum L.) an agro-economically important food crop in the world, is sensitive to many fungal pathogens including Rhizoctonia solani (AG-3), the causal agent of stem and root rot diseases. Chitinase and glucanase are cell wall degrading enzymes which have been shown to have high antifungal activity against a wide range of phytopathogenic fungi. In the present study, plasmid pBIKE3 harboring a double-gene cassette containing the chitinase (chit42) and b-1,3-glucanase (bgn13.1) genes was constructed. In this construct, the chit42 gene is located between the CaMV 35S promoter and nos terminator derived from pBI121, while the bgn13.1 gene is downstream of a modified CaMV 35S promoter, followed by the nos terminator both of which were derived from the pRTL plasmid. Micro-tubers of potato plants (the Savalan cultivar) were transformed with the pBIKE3 construct via the Agrobacterium delivery system. Integration of these two genes into the potato genome and their expression at the transcriptional level was confirmed by polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR). The radial diffusion assay showed that the heterologous expressed chitinase and glucanase enzymes demonstrated antifungal activity on R. solani (AG-3).
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نویسندگان مقاله کسری اصفهانی |
national institute for genetic engineering and biotechnology nigeb , p.o. box 14965 161, tehran, i.r. iran

مصطفی مطلبی |
national institute for genetic engineering and biotechnology nigeb , p.o. box 14965 161, tehran, i.r. iran

محمدرضا زمانی | mohammad reza
national institute for genetic engineering and biotechnology nigeb , p.o. box 14965 161, tehran, i.r. iran

هاله هاشمی سهی | hashemi sohi
national institute for genetic engineering and biotechnology nigeb , p.o. box 14965 161, tehran, i.r. iran

عصمت جورابچی |
national institute for genetic engineering and biotechnology nigeb , p.o. box 14965 161, tehran, i.r. iran

نشانی اینترنتی http://www.ijbiotech.com/article_7104_27420714a7214f69645952f19e7fbb7e.pdf
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