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Iranian Journal of Biotechnology، جلد ۸، شماره ۲، صفحات ۱۰۳-۱۱۱
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عنوان انگلیسی Characterization of an a-Amylase with Broad Temperature Activity from an Acid-Neutralizing Bacillus cereus Strain
چکیده انگلیسی مقاله Bacillus sp. GUF8, isolated from acidic soil samples of a tea farm was identified as Bacillus cereus, based on 16S rDNA sequencing and standard bacterial identification methods. Following optimization of enzyme production, the resulting α-amylase was purified by acetone precipitation and ion exchange chromatography. Consequently, thermostability and kinetic parameters of the purified enzyme were determined. The temperature profile of the enzyme indicated a very broad temperature range (from 10 to 70°C) with 50°C representing the optimum temperature for enzyme activity, which is different from those of the known Bacillus a-amylases. This enzyme was optimally active at pH 6.0 and retained 75 and 50% of its maximal activity at pH 8.0 and 9.0, respectively. It was also strongly inhibited by Zn2+ and partially inhibited by Ni2+ and ethylenediaminetetraacetic acid (EDTA). The a-amylase enzyme was found to hydrolyze starch forming various maltooligosaccharides, such as maltose (G2) and maltopentaose (G5) as major products.
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نویسندگان مقاله عطیه مهدوی |
department of biology, faculty of science, university of guilan, p.o. box 41335-1914, rasht, iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)

رضا حسن ساجدی | hassan sajedi
department of biology, faculty of science, university of guilan, p.o. box 41335-1914, rasht, iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)

مهدی رسا |
department of biology, faculty of science, university of guilan, p.o. box 41335-1914, rasht, iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)

وهاب جعفریان |
department of biology, faculty of science, university of guilan, p.o. box 41335-1914, rasht, iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)

نشانی اینترنتی http://www.ijbiotech.com/article_7096_5ccaec9332530e0dcfa232ec4f358a6c.pdf
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