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Iranian Journal of Biotechnology، جلد ۸، شماره ۱، صفحات ۴۶-۴۹
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عنوان انگلیسی Molecular Detection of Lipase A gene in Putative Bacillus subtilis Strains Isolated from Soil
چکیده انگلیسی مقاله The present study was undertaken to screen the soil samples collected in Iran for the presence of the Bacillus subtilis lipase A gene. The bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and the polymerase chain reaction (PCR). Only four colonies were identified as putative B. subtilis strains and all contained the lipase A gene. However, the intensities of the DNA bands were different and correlated with the differences obtained from the biochemical tests. Polymorphism of the lipase gene was also determined in samples using SSCP assay. In conclusion, this study demonstrates an easy and reliable method for detection of the lipase gene in B. subtilis strains. Further screening of the soil by this method will enable the detection and identification of industrially more favorable lipases.
کلیدواژه‌های انگلیسی مقاله Lipase A gene,PCR,Bacillus subtilis,Soil,detection
نویسندگان مقاله حمید میرمحمدصادقی | mir mohammad sadeghi
department of biotechnology and isfahan pharmaceutical research center, school of pharmacy, isfahan university of medical sciences, isfahan, i.r. iran and applied physiology research center, isfahan university of medical sciences, isfahan, i.r. iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

محمد ربانی |
department of biotechnology and isfahan pharmaceutical research center, school of pharmacy, isfahan university of medical sciences, isfahan, i.r. iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

فاطمه موذن |
department of biotechnology and isfahan pharmaceutical research center, school of pharmacy, isfahan university of medical sciences, isfahan, i.r. iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

ساره همامی |
department of biotechnology and isfahan pharmaceutical research center, school of pharmacy, isfahan university of medical sciences, isfahan, i.r. iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

نشانی اینترنتی http://www.ijbiotech.com/article_7095_8b3deb50385951db5f5b4d31665798ce.pdf
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