این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Biotechnology، جلد ۷، شماره ۲، صفحات ۱۱۲-۱۱۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Construction of Recombinant Vectors Containing Clavulanic Acid Antibiotic Regulatory Gene, claR, Isolated of Streptomyces clavuligerus Strains
چکیده انگلیسی مقاله The claR of Streptomyces clavuligerus in the clavulanic acid gene cluster encodes a transcriptional regulator that controls clavulanic acid biosynthesis. The main goal of this study was isolation and molecular detection of the claR gene and its cloning in the Streptomyces specific vector (pMA:: hyg). By cinsideration of the claR gene’s start codon, the specific primers were designed. After genomic DNA extraction from S. clavuligerus, the claR gene was amplified by Polymerase Chain Reaction (PCR). The structure of the amplified claR was confirmed by nested-PCR, PCR-restriction fragment length polymorphism (PCR-RFLP), and sequencing. A ligation mixture was prepared with the isolated claR gene and cut pMA::hyg vector. Escherichia coli competent cells were finally transformed with the ligation mixture. Presence of the recombinant vector in the transformed colonies was then confirmed by the colony-PCR procedure. The claR gene was also isolated from S. clavuligerus DSM41826, cloned and sequenced in the same manner. The pMA::hyg vector is a shuttle vector, which exists as a multicopy plasmid in E. coli, and as an integrative plasmid in Streptomyces. Therefore, the newly constructed vectors of this study can be regarded as an appropriate tool for site-directed mutagenesis and gene replacement strategies in S. clavuligerus.
کلیدواژه‌های انگلیسی مقاله Clavulanic acid,S. clavuligerus,pMA hyg vector,claR gene
نویسندگان مقاله فرانک قاضی شعرباف | ghazi sherbaf
department of biology, faculty of sciences, university of isfahan, p.o. box 81746-73441, isfahan, i.r. iran
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

زهره حجتی |
department of biology, faculty of sciences, university of isfahan, p.o. box 81746-73441, isfahan, i.r. iran
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

مجید متولی باشی | motovali bashi
department of biology, faculty of sciences, university of isfahan, p.o. box 81746-73441, isfahan, i.r. iran
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

نشانی اینترنتی http://www.ijbiotech.com/article_7092_0f14c861fc538d882c8f1aa3b10f4f51.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات