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Iranian Journal of Biotechnology، جلد ۶، شماره ۴، صفحات ۱۹۹-۲۰۶
عنوان فارسی
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عنوان انگلیسی Phenotypic and Molecular Screening of Tomato Germplasm for Resistance to Tomato Yellow Leaf Curl Virus
چکیده انگلیسی مقاله Tomato yellow leaf curl virus (TYLCV) is a major tomato virus in tropical and subtropical regions. In this study, 134 accessions of Solanum lycopersicum and six accessions of Solanum peruvianum were assessed for resistance to an Iranian isolate of TYLCV. Plants were inoculated using whiteflies (Bemisia tabaci) and the reaction of plants was evaluated based on either disease symptoms or viral DNA amplification. All accessions of S. lycopersicum had demonstrated various degrees of disease symptoms. However, all six accessions of S. peruvianum were resistant and remained symptomless. Phenotypic evaluation was confirmed by amplification of a 670bp TYLCV DNA fragment in all tested accessions of S. lycopersicum. Based on both phenotypic and molecular evaluations, no accession provided complete resistance to TYLCV, whereas nine accessions were assessed as tolerant. The high level of resistance noted in whitefly inoculated accessions of S. peruvianum was not observed in graft inoculated plants of these accessions. The TYLCV DNA fragment was detected five weeks post-inoculation when plants were inoculated by grafting. These results suggested that accessions of S. peruvianum may be merely resistant to vector inoculation of TYLCV.
کلیدواژه‌های انگلیسی مقاله Molecular screening,Tomato,TYLCV,Virus resistance
نویسندگان مقاله عبدالباسط عزیزی |
national plant gene-bank, seed and plant improvement institute, mahdasht road, p.o box 31585-4119, karaj, iran and department of plant pathology, faculty of agriculture,tarbiat modares university, p.o. box 14115-326 tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

جواد مظفری |
national plant gene-bank, seed and plant improvement institute, mahdasht road, p.o box 31585-4119, karaj, iran

مسعود شمس بخش | shams bakhsh
department of plant pathology, faculty of agriculture,tarbiat modares university, p.o. box 14115-326 tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://www.ijbiotech.com/article_7037_dd9cda0e0df121a5d3204158dab23f4e.pdf
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