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Iranian Journal of Biotechnology، جلد ۶، شماره ۱، صفحات ۳۶-۴۴
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عنوان انگلیسی Interferon Resistance of Hepatitis C Virus Genotypes 1a/1b: Relationship to Structural E2 Gene Quasispecies Mutations
چکیده انگلیسی مقاله Hepatitis C virus (HCV) envelope glycoprotein-2 (E2) inhibits the interferon (IFN)–induced, double –stranded RNA activated protein kinase (PKR) via PKR eukaryotic initiation factor-2α phosphorylation homology domain (PePHD). Present study examined the genetic variability of the PePHD in patients receiving interferon therapy. The PePHD region from HCV genotype 1a/1b infected patients receiving IFN was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and analyzed using bidirectionaly sequencing. The PePHD sequence was different in pretreatment isolates from three months treated patients. It was shown that the major PePHD quasispecies could change after three months IFN therapy and in one patient; the major PePHD quasispecies could change after six months IFN therapy. These mutations were occurred at codons 665, 666 and 667 of followed-up samples and at codons 660, 661, 666 and 670 of randomly treated patients. Some of these mutations were similar to those reported in previous studies. Other mutations were also detected in upstream and downstream regions of PePHD which may have influenced the structure, conformation and configuration of this region and thereby suppressing PePHD inhibitory properties. In conclusion our data suggested that HCV E2 PePHD may play an important role in determining the interferon response among Iranian HCV infected patients.
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نویسندگان مقاله مریم هنردوست |
department of virology, faculty of medical sciences, tarbiat modares university, tehran, p.o. box 14155-111, i.r. iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

فرزانه صباحی |
department of virology, faculty of medical sciences, tarbiat modares university, tehran, p.o. box 14155-111, i.r. iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

صمد امینی باویل علیایی | amini bavil olyaee
biotechnology department, pasteur institute of tehran, p.o. box 13164, i.r. iran 3digestive diseases research center, shariati hospital, tehran, p.o. box 14117-13135, i.r. iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

فریدا بهزادیان |
department of virology, faculty of medical sciences, tarbiat modares university, tehran, p.o. box 14155-111, i.r. iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

شاهین مرآت |
digestive diseases research center, shariati hospital, tehran, p.o. box 14117-13135, i.r. iran

رضا ملک زاده |
digestive diseases research center, shariati hospital, tehran, p.o. box 14117-13135, i.r. iran

نشانی اینترنتی http://www.ijbiotech.com/article_7062_4cef073e0ce79458e1871bf7e2be3a10.pdf
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