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Iranian Journal of Biotechnology، جلد ۵، شماره ۲، صفحات ۱۰۰-۱۰۴
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عنوان انگلیسی A Protocol for Mass Production of Rosa hybrida cv. Iceberg Through in vitro Propagation
چکیده انگلیسی مقاله Interactive effect of plant growth regulators 6-Benzylaminopurine (BAP) (0, 2, 4 and 8 µM) and 1-Naphtalene acetic acid (NAA) (0, 0.05, 0.25 and 0.5 µM) in Van der Salm (VS) medium was used to optimize in vitro propagation of Rosa hybrida cv. Iceberg. Shoot proliferation and number of new leaves were measured as growth indicators. As the concentration of BAP was raised, growth rate increased with all of the above NAA concentrations. However, the highest number of axillary shoots and new leaves were produced with 4 µM BAP, which was considered the optimal level. A multiplication rate of 10 folds with a maximum number of axillary shoots (10.1) and new leaves per explant (25) were obtained in the medium containing 4 µM BAP plus 0.5 µM NAA. In vitro-derived shoots were used to investigate root initiation and growth by lowering the concentration of VS mineral salts and vitamins. Three strengths of VS (full, 1/2 and 1/4) were compared in semi-solid and liquid medium. The average number of roots (4.35) and root length (0.82 cm) were significantly higher in 1/4 strength VS. The highest percentage of rooting (93.33%) and number of roots (4.45) were significantly higher in semi-solid than liquid medium. The regenerated plantlets were successfully transferred to soil and the survival rates of the rooted plantlets transferred to soil were 70% and 90% in plants treated with semi-sold and liquid media, respectively
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نویسندگان مقاله پگاه خسروی |
department of crop sciences, university of sari, sari, i.r. iran

مریم جعفرخانی کرمانی | jafarkhani kermani
department of tissue culture and gene transformation, agricultural biotechnology research institute of iran, p.o. box 31535-1897, karaj, i.r. iran

قربانعلی نعمت زاده | gorban ali
department of crop sciences, university of sari, sari, i.r. iran

محمدرضا بی همتا | mohammad reza
department of plant breeding, university of tehran, p.o. box 31587-8710, karaj, i.r. iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://www.ijbiotech.com/article_7014_29f3492c705de224e21062a8ab7166f9.pdf
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